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1979 Onderstepoort Journal of Veterinary Research, Volume 46, 1979: Recent submissions

  • Studies on Haemonchus contortus. II. The effect of abomasal nematodes on subsequent challenge with H. contortus 
    Reinecke, R.K.; Snyman, H. Maria; Seaman, Helga; Steyn, P.J.J.; Bigalke, R.D.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B.; Cameron, Colin McKenzie; Gilchrist, Frances M.C. (Published by The Government Printer, Pretoria, 1979)
    Fifty-thousand infective larvae of Trichostrongylus axei dosed to 7-9-month-old Merinos protected them against a subsequent challenge with 50 000 infective larvae of Haemonchus contortus. Protection varied with the period ...
  • Actinobacillus seminis infection in sheep in the Republic of South Africa. II. Incidence and geographical distribution 
    Van Tonder, E.M.; Steyn, P.J.J.; Bigalke, R.D.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B.; Cameron, Colin McKenzie; Gilchrist, Frances M.C. (Published by The Government Printer, Pretoria, 1979)
    To obtain information on the incidence and distribution of Actinobacillus seminis infection in the Republic of South Africa, a clinical and serological survey was carried out on 409 farms situated in 29 districts. All rams ...
  • Parasites of domestic and wild animals in South Africa. XIII. The seasonal incidence of adult ticks (Acarina : Ixodidae) on cattle in the northern Transvaal 
    Horak, Ivan Gerard; Londt, J.G.H.; De Villiers, I.L.; Steyn, P.J.J.; Bigalke, R.D.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B.; Cameron, Colin McKenzie; Gilchrist, Frances M.C. (Published by The Government Printer, Pretoria, 1979)
    Ticks were collected over a period of 13 months, at approximately fortnightly intervals, from 6 Africaner oxen kept on the farm Nylsvley in the Naboomspruit District. The 8 species of Ixodidae collected were, in order of ...
  • Actinobacillus seminis infection in sheep in the Republic of South Africa. I. Identification of the problem 
    Van Tonder, E.M.; Steyn, P.J.J.; Bigalke, R.D.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B.; Cameron, Colin McKenzie; Gilchrist, Frances M.C. (Published by The Government Printer, Pretoria, 1979)
    A clinical palpation and semen smear examination of 647 rams submitted to the Regional Veterinary Laboratory during 1967 revealed that 42 (6,5%) of these animals had clinical epididymitis or orchitis, 6 (0,9%) showed other ...
  • Parasites of domestic and wild animals in South Africa. XII. Artificial transmission of nematodes from blesbok and impala to sheep, goats and cattle 
    Horak, Ivan Gerard; Steyn, P.J.J.; Bigalke, R.D.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B.; Gilchrist, Frances M.C.; Cameron, Colin McKenzie (Published by The Government Printer, Pretoria, 1979)
    Sheep were successfully infested artificially with the larvae of Haemonchus contortus, Trichostrongylus axei, Trichostrongylus falculatus and Impalaja nudicollis, cultured from the faeces of blesbok naturally infested with ...
  • Subcutaneous and pulmonary emphysema as complications of bovine ephemeral fever 
    Theodoridis, A.; Coetzer, Jacobus A.W.; Steyn, P.J.J.; Bigalke, R.D.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B.; Cameron, Colin McKenzie; Gilchrist, Frances M.C. (Published by The Government Printer, Pretoria, 1979)
    Subcutaneous and pulmonary emphysema was observed in some cattle on farms on which outbreaks of bovine ephemeral fever (BEF) occurred. BEF virus was isolated in baby hamsters from one of the cases and cattle were injected ...
  • Viruses isolated from Culicoides midges in South Africa during unsuccessful attempts to isolate bovine ephemeral fever virus 
    Theodoridis, A.; Nevill, E.M.; Els, H.J.; Boshoff, S.T.; Steyn, P.J.J.; Bigalke, R.D.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B.; Cameron, Colin McKenzie; Gilchrist, Frances M.C. (Published by The Government Printer, Pretoria, 1979)
    Five viruses, unrelated to bovine ephemeral fever virus (BEFV), were isolated from Culicoides biting-midges collected during the summer months of the years 1968-69 and 1969-70 near a cattle herd in which cases of BEF ...
  • Isolation and characterization of antibodies to Clostridium perfringens epsilon toxin from hyperimmune horse serum 
    Worthington, R.W.; Mulders, M.S.G.; Steyn, P.J.J.; Bigalke, R.D.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B.; Cameron, Colin McKenzie; Gilchrist, Frances M.C. (Published by The Government Printer, Pretoria, 1979)
    Antibodies against epsilon toxin were isolated from hyperimmune horse serum by affinity chromatography. Purified epsilon prototoxin covalently bound to Affigel 202 was used as immunosorbent, and antibodies were eluted with ...
  • The effect of temperature on the frons width in males of Stomoxys calcitrans Linnaeus (Diptera : Muscidae) 
    Sutherland, B.; Steyn, P.J.J.; Bigalke, R.D.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B.; Cameron, Colin McKenzie; Gilchrist, Frances M.C. (Published by The Government Printer, Pretoria, 1979)
    Laboratory experiments established that males of Stomoxys calcitrans, raised at a high temperature (30°C), had frons widths that were very significantly smaller (P=0,01) than those of males raised at a lower temperature ...
  • Aerospora of an Eragrostis curvula pasture in South Africa 
    Van der Merwe, W.J.J.; Eicker, A.; Marasas, Walter F.O. (Walter Friedrich Otto); Kellerman, T. Stephanus; Steyn, P.J.J.; Bigalke, R.D.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B.; Cameron, Colin McKenzie; Gilchrist, Frances M.C. (Published by The Government Printer, Pretoria, 1979)
    A qualitative and quantitative survey was made of the aerospora above an Eragrostis curvula (Schrad.) Nees pasture. The pasture, which lay adjacent to a field of Panicum coloratum L. where an outbreak of ovine photosensitivity ...
  • Production and application of a live Salmonella gallinarum vaccine 
    Cameron, Colin McKenzie; Buys, S.B.; Steyn, P.J.J.; Bigalke, R.D.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B.; Cameron, Colin McKenzie; Gilchrist, Frances M.C. (Published by The Government Printer, Pretoria, 1979)
    The production and application of a freeze-dried Salmonella gallinarum vaccine are described in this report. The vaccine is stable when kept at 4 °C and a single injection elicits a good immunity for 2 months, though its ...
  • Factors affecting the bluetongue virus neutralizing antibody response and the reaction between virus and antibody 
    Van der Walt, N.T.; Steyn, P.J.J.; Bigalke, R.D.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B.; Cameron, Colin McKenzie; Gilchrist, Frances M.C. (Published by The Government Printer, Pretoria, 1979)
    A study was made of different aspects of the bluetongue virus neutralizing antibody response and the reaction between the virus and antibody. Optimum neutralization was obtained in a 2 mM Tris-HCl buffer, pH 9,0, at a ...
  • Descriptions of the adults and pupa of Culicoides hildae n. sp. from the Republic of South Africa (Diptera : Ceratopogonidae) 
    Cornet, M.; Nevill, E.M.; Steyn, P.J.J.; Bigalke, R.D.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B.; Cameron, Colin McKenzie; Gilchrist, Frances M.C. (Published by The Government Printer, Pretoria, 1979)
    A new species of biting midge, Culicoides hildae, is described from males, females and pupal exuviae collected in the Orange Free State, Republic of South Africa.
  • Micromorphology of the skin (epidermis, dermis, subcutis) of the dog 
    Schwarz, R.; Le Roux, J.M.W.; Schaller, R.; Neurand, K.; Steyn, P.J.J.; Bigalke, R.D.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B.; Cameron, Colin McKenzie; Gilchrist, Frances M.C. (Published by The Government Printer, Pretoria, 1979)
    Grossly, the skin of the dog is characterized by varying thicknesses, density of the hair coat and the implantation angle of the hairs. Multiple folds varying in shape are formed by the epidermis and the papillary layer ...
  • The nucleic acid and proteins of epizootic haemorrhagic disease virus 
    Huismans, H. (Henk), 1942-; Bremer, C.W.; Barber, T.L.; Steyn, P.J.J.; Bigalke, R.D.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B.; Cameron, Colin McKenzie; Gilchrist, Frances M.C. (Published by The Government Printer, Pretoria, 1979)
    Purified epizootic haemorrhagic disease virus (EHDV) was shown to contain 10 double-stranded RNA segments and a double-layered protein capsid with 4 major and 4 minor polypeptides. The virus differed from bluetongue virus ...
  • The use of frequency diagrams in the survey of resistance to pesticides in ticks in southern Africa 
    Solomon, K.R.; Baker, Maureen K.; Heyne, Heloise; Van Kleef, Jacqueline; Steyn, P.J.J.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B.; Bigalke, R.D.; Cameron, Colin McKenzie; Gilchrist, Frances M.C. (Published by The Government Printer, Pretoria, 1979)
    A method whereby resistance data can be analysed by means of frequency distributions is described. This method established that Rhipicephalus appendiculatus and R. evertsi evertsi show either little or only developing ...
  • Lymph node pathology in Trypanosoma brucei-infected sheep 
    Barrowman, P.R.; Roos, J.A.; Steyn, P.J.J.; Bigalke, R.D.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B.; Gilchrist, Frances M.C.; Cameron, Colin McKenzie (Published by The Government Printer, Pretoria, 1979)
    The temporal sequence of morphological changes occurring in the prefemoral lymph nodes of sheep experimentally infected with Trypanosoma brucei was studied at weekly intervals during the course of infection. Histopathological ...
  • Genetic and physiological characterization of temperature-sensitive mutants of bluetongue virus 
    Shipham, S.O.; De la Rey, Marlene; Steyn, P.J.J.; Bigalke, R.D.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B.; Cameron, Colin McKenzie; Gilchrist, Frances M.C. (Published by The Government Printer, Pretoria, 1979)
    Complementation studies were carried out, using temperature-sensitive (t-s) mutants of bluetongue virus (BTV). The results proved to be inconclusive as only low indices of complementation were obtained. No discrepancy was ...
  • Composition and evaluation of the efficacy of a Staphylococcus aureus vaccine 
    Cameron, Colin McKenzie; Fuls, W.J.P.; Botha, Wilna F.; Steyn, P.J.J.; Bigalke, R.D.; Morren, A.J.; Verster, Anna J.M.; Verwoerd, Daniel Wynand; Walker, Jane B.; Cameron, Colin McKenzie; Gilchrist, Frances M.C. (Published by The Government Printer, Pretoria, 1979)
    An alum-precipitated Staphylococcus aureus vaccine, composed of a formalin-inactivated whole culture of a strain which produces Smith surface antigen and combined with the whole culture of a highly toxigenic strain, was ...