Genetic and physiological characterization of temperature-sensitive mutants of bluetongue virus

Loading...
Thumbnail Image

Date

Authors

Shipham, S.O.
De la Rey, Marlene

Journal Title

Journal ISSN

Volume Title

Publisher

Published by The Government Printer, Pretoria

Abstract

Complementation studies were carried out, using temperature-sensitive (t-s) mutants of bluetongue virus (BTV). The results proved to be inconclusive as only low indices of complementation were obtained. No discrepancy was found between the previous classification of these mutants in 6 recombination classes and the complementation data recorded. In general, the t-s mutants require a latent growth period of 16-20 h at 28 °C and maximum titres can be demonstrated 40-48 h post-infection. One mutant, (F211), however, consistently had a growth lag phase of 32 h. Mutants of the 6 recombination groups were further classified into 2 groups by temperature-shift studies. One class of mutants expressed their t-s lesion prior to 24 h and the other class only after 24 h post-infection. Mutant F73 was found to be defective in its ability to synthesize ssRNA at a late stage in the replication cycle at the non-permissive temperature.

Description

This article has been scanned in colour with a HP Scanjet 5590; 300dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-Format.

Keywords

Veterinary medicine

Sustainable Development Goals

Citation

Shipham, SO, De la Rey, M 1979, 'Genetic and physiological characterization of temperature-sensitive mutants of bluetongue virus’, Onderstepoort Journal of Veterinary Research, vol. 46, no. 2, pp. 87-94.