Onderstepoort Journal of Veterinary Research

Onderstepoort Journal of Veterinary Research

 

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  • Biggs, H.C.; Langenhoven, J.W.; Bigalke, R.D. (Pretoria : Government Printer, 1984)
    The seasonal prevalence of ixodid ticks was determined over a 2-year period by the weekly removal from at least 5 cattle of all adult ticks and the immature stages of Rhipicephalus evertsi mimeticus in the ears. The highest ...
  • Anonymous; Bigalke, R.D. (Pretoria : Government Printer, 1984)
  • Anonymous; Bigalke, R.D. (Pretoria : Government Printer, 1984)
  • Van de Pypekamp, H.E.; Prozesky, L.; Bigalke, R.D. (Published by the Government Printer, Pretoria., 1987)
    Heartwater is a frequently fatal tick-borne disease of ruminants caused by Cowdria ruminantium. In domestic ruminants the incubation period varies considerably and depends on the route of infection, virulence of the ...
  • Scott, G.R.; Bigalke, R.D. (Published by the Government Printer, Pretoria., 1987)
    A fresh pragmatic classification of the rickettsias has been derived by applying the simplest techniques of numerical taxonomy. One order, the Rickettsiales, containing 3 families, the Rickettsiaceae, Phagosomaphilaceae, and ...
  • Ambrosio, R.E.; Du Plessis, J.L.; Bezuidenhout, J.D.; Bigalke, R.D. (Published by the Government Printer, Pretoria., 1987)
    Genomic libraries of the Welgevonden and Kwanyanga isolates of Cowdria ruminantium have been constructed in an expression vector. These libraries contain approximately 4 x 10⁵ and 3 x 10⁵ recombinants respectively.
  • Neitz, A.W.H.; Vermeulen, N.M.J.; Bigalke, R.D. (Published by the Government Printer, Pretoria., 1987)
    Immunoselective methods with special reference to immunoadsorbent affinity chromatography as a means for the isolation of Cowdria ruminantium are reviewed. Attention is given to the source of the organism, immunization, ...
  • Vermeulen, N.M.J.; Neitz, A.W.H.; Viljoen, G.J.; Bigalke, R.D. (Published by the Government Printer, Pretoria., 1987)
    This review covers the isolation of Cowdria ruminantium by lectin cellular affinity chromatography from different Amblyomma hebraeum sources. Cellular affinity chromatography has been reviewed with special attention being ...
  • Neitz, A.W.H.; Vermeulen, N.M.J.; Viljoen, G.J.; Bigalke, R.D. (Published by the Government Printer, Pretoria., 1987)
    The isolation of Cowdria ruminantium by differential and isopycnic density gradient centrifugation is reviewed with special reference to the suitability of Percoll as density gradient medium. Infected sheep brain, Amblyomma ...
  • Williams, J.C.; Vodkin, M.H.; Bigalke, R.D. (Published by the Government Printer, Pretoria., 1987)
    Chlamydial and rickettsial diseases pose a hazard to man and to domesticated and wild animals. The virulence mechanisms which aid the establishment of these obligate intracellular parasites in the eukaryotic host are ...
  • Bezuidenhout, J.D.; Bigalke, R.D. (Published by the Government Printer, Pretoria., 1987)
    Attempts were made to grow 4 isolates of Cowdria ruminantium in cell lines. Three of these isolates, viz. Ball 3, Welgevonden and Kwanyanga, could be cultivated in a calf endothelial cell line, but experiments with the ...
  • Logan, L.L.; Whyard, T.C.; Quintero, J.C.; Mebus, C.A.; Bigalke, R.D. (Published by the Government Printer, Pretoria., 1987)
    The sequential development of C. ruminantium (Kwanyanga and Kumm isolates) was followed in caprine leukocyte cultures by light microscopy, direct immunofluorescent microscopy (DFA), indirect immunofluorescent microscopy ...
  • Prozesky, L.; Du Plessis, J.L.; Bigalke, R.D. (Published by the Government Printer, Pretoria., 1987)
    Various aspects of the development and life cycle of Cowdria ruminantium are discussed. C. ruminantium is transmitted transstadially by certain Amblyomma species. Apparently organisms initially develop in the gut epithelial ...
  • Logan, L.L.; Bigalke, R.D. (Published by the Government Printer, Pretoria., 1987)
    Blood collected in either sodium heparin or disodium edetate vacutainers from febrile goats infected with 4 isolates of Cowdria ruminantium and cryopreserved with 10% dimethyl sulphoxide at -70⁰C and -196⁰C was an effective ...
  • Yunker, C.E.; Kocan, K.M.; Norval, R.A.I.; Burridge, M.J.; Bigalke, R.D. (Published by the Government Printer, Pretoria., 1987)
    Mallory's phloxine-methylene blue stain was used to differentiate colonies of Cowdria ruminantium in midgut epithelial cells of nymphal Amblyomma hebraeum that had been infected as larvae. Gut tissues were collected from ...
  • Kocan, K.M.; Bezuidenhout, J.D.; Bigalke, R.D. (Published by the Government Printer, Pretoria., 1987)
    The morphology and development of Cowdria ruminantium have been studied in Amblyomma hebraeum and A. variegatum. Colonies of C. ruminantium have so far been demonstrated microscopically in gut, salivary gland cells, ...
  • Prozesky, L.; Bigalke, R.D. (Published by the Government Printer, Pretoria., 1987)
    The morphology of Cowdria ruminantium is described and its staining characteristics in the vertebrate host and in vitro are summarized. Morphologically, the organisms are characterized in the cytoplasm of endothelial cells, ...
  • Bezuidenhout, J.D.; Provost, A.; Bigalke, R.D. (Published by the Government Printer, Pretoria., 1987)
    The first written record of what probably could have been heartwater originates from South Africa and dates back to 1838. Since then, the disease was described from almost all the African countries south of the Sahara ...
  • Van der Merwe, F.J.; Bigalke, R.D. (Published by the Government Printer, Pretoria., 1987)
  • Verwoerd, Daniel Wynand; Verwoerd, Daniel Wynand (Published by the Agricultural Research Council, Onderstepoort Veterinary Institute, 1997)
    This volume of the journal is dedicated to Max Sterne in recognition of his contributions to veterinary bacteriology.

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