Browsing 1987 Onderstepoort Journal of Veterinary Research, Volume 54, 1987 by Issue Date

Browsing 1987 Onderstepoort Journal of Veterinary Research, Volume 54, 1987 by Issue Date

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  • Heartwater : Past, Present and Future. Workshop (1986 : Kruger National Park, South Africa); Purnell, R.E.; Bigalke, R.D. (Published by the Government Printer, Pretoria., 1987)
    As a result of a personal survey in 1982, interviewing veterinarians from Louis Trichardt to Uitenhage, it was concluded that bovine heartwater is currently regarded as the most serious tick-borne disease problem in South ...
  • Williams, J.C.; Vodkin, M.H.; Bigalke, R.D. (Published by the Government Printer, Pretoria., 1987)
    Chlamydial and rickettsial diseases pose a hazard to man and to domesticated and wild animals. The virulence mechanisms which aid the establishment of these obligate intracellular parasites in the eukaryotic host are ...
  • Logan, L.L.; Whyard, T.C.; Quintero, J.C.; Mebus, C.A.; Bigalke, R.D. (Published by the Government Printer, Pretoria., 1987)
    The sequential development of C. ruminantium (Kwanyanga and Kumm isolates) was followed in caprine leukocyte cultures by light microscopy, direct immunofluorescent microscopy (DFA), indirect immunofluorescent microscopy ...
  • Bezuidenhout, J.D.; Bigalke, R.D. (Published by the Government Printer, Pretoria., 1987)
    Attempts were made to grow 4 isolates of Cowdria ruminantium in cell lines. Three of these isolates, viz. Ball 3, Welgevonden and Kwanyanga, could be cultivated in a calf endothelial cell line, but experiments with the ...
  • Heartwater : Past, Present and Future. Workshop (1986 : Kruger National Park, South Africa); Oberem, P.T.; Bezuidenhout, J. Dürr; Bigalke, R.D. (Published by the Government Printer, Pretoria., 1987)
    The events leading to the production of an effective vaccine against heartwater are summarized. The production techniques used at Onderstepoort to produce and control the quality of the infected blood and nymph suspension ...
  • Heartwater : Past, Present and Future. Workshop (1986 : Kruger National Park, South Africa); McHardy, N.; MacKenzie, P.K.I.; Bigalke, R.D. (Published by the Government Printer, Pretoria., 1987)
    Heartwater is a tick-transmitted rickettsial infection of ruminants, caused by Cowdria ruminantium. The tetracyclines are the only compounds available for therapy of the disease. A screen, using mice infected with C. ...
  • Jansen, B.C.; Hayes, M; Bigalke, R.D. (Published by the Government Printer, Pretoria., 1987)
    Ten bacterial species were isolated from areas on the skin of Merino sheep affected by a chronic scaly condition leading to retarded wool growth. The growth requirements of the bacteria were studied and it was shown that ...
  • Kleynhans, K.P.N.; Bigalke, R.D. (Published by the Government Printer, Pretoria., 1987)
    The adults, puparium and 3rd instar larva of a dung-breeding fly, Musca nevilli sp. nov. are described in the subgenus Eumusca. The adults are characterized by 4 dark postsutural mesonotal vittae, 1-2 bristles dorsally on ...
  • Verster, Anna; Tustin, R.C.; Bigalke, R.D. (Published by the Government Printer, Pretoria., 1987)
    A trial with 200 ewes and 127 lambs showed that vaccination of lambs with 2 doses of Oncosphere Secretory Antigen (OSA) 4 weeks apart, at either 4-8 and 8-12 weeks or at 12-16 and 16-20 weeks of age, was equally effective ...
  • Meiswinkel, R.; Bigalke, R.D. (Published by the Government Printer, Pretoria., 1987)
    The discovery of Culicoides kanagai in South Africa represents a new record for this species. The female is redescribed, and the male is described for the first time. Culicoides (A.) dasyops Clastrier, 1958 is shown to be ...
  • Heartwater : Past, Present and Future. Workshop (1986 : Kruger National Park, South Africa); Du Plessis, J.L.; Malan, Letitia; Bigalke, R.D. (Published by the Government Printer, Pretoria., 1987)
    The preparation of the antigen, details of the reagents, the titration of the antispecies conjugates and the execution of the indirect fluorescent antibody test are described. The sensitivity and specificity of the test ...
  • Heartwater : Past, Present and Future. Workshop (1986 : Kruger National Park, South Africa); Du Plessis, J.L.; Malan, Letitia; Kowalski, Z.E.; Bigalke, R.D. (Published by the Government Printer, Pretoria., 1987)
    Hypotheses on the pathogenesis of heartwater, which have been published so far, are briefly reviewed. Attempts were made at counteracting the effects of vaso-active substances released by mast cells by treating mice infected ...
  • Barham, W.T.; Schoonbee, H.J.; Visser, J.G.J.; Bigalke, R.D. (Published by the Government Printer, Pretoria., 1987)
    Procedures to narcotize fish by means of alternating or direct electrical currents are described and a method of evaluating the narcotic effects on fish of electrical currents is detailed. The role of fish length on the ...
  • Heartwater : Past, Present and Future. Workshop (1986 : Kruger National Park, South Africa); Holland, C.J.; Logan, L.L.; Mebus, C.A.; Ristic, M.; Bigalke, R.D. (Published by the Government Printer, Pretoria., 1987)
    Although Cowdria ruminantium has been classified within the tribe Ehrlichia of the family Rickettsiaceae, there has been no extensive study conducted regarding its antigenic relationship to members of the genus Ehrlichia, ...
  • Prozesky, L.; Du Plessis, J.L.; Bigalke, R.D. (Published by the Government Printer, Pretoria., 1987)
    Various aspects of the development and life cycle of Cowdria ruminantium are discussed. C. ruminantium is transmitted transstadially by certain Amblyomma species. Apparently organisms initially develop in the gut epithelial ...
  • Vermeulen, N.M.J.; Neitz, A.W.H.; Viljoen, G.J.; Bigalke, R.D. (Published by the Government Printer, Pretoria., 1987)
    This review covers the isolation of Cowdria ruminantium by lectin cellular affinity chromatography from different Amblyomma hebraeum sources. Cellular affinity chromatography has been reviewed with special attention being ...
  • Scott, G.R.; Bigalke, R.D. (Published by the Government Printer, Pretoria., 1987)
    A fresh pragmatic classification of the rickettsias has been derived by applying the simplest techniques of numerical taxonomy. One order, the Rickettsiales, containing 3 families, the Rickettsiaceae, Phagosomaphilaceae, and ...
  • Ambrosio, R.E.; Du Plessis, J.L.; Bezuidenhout, J.D.; Bigalke, R.D. (Published by the Government Printer, Pretoria., 1987)
    Genomic libraries of the Welgevonden and Kwanyanga isolates of Cowdria ruminantium have been constructed in an expression vector. These libraries contain approximately 4 x 10⁵ and 3 x 10⁵ recombinants respectively.
  • Heartwater : Past, Present and Future. Workshop (1986 : Kruger National Park, South Africa); Bezuidenhout, J. Dürr; Olivier, J.A.; Gruss, B.; Badenhorst, J.V.; Bigalke, R.D. (Published by the Government Printer, Pretoria., 1987)
    The efficacy of subcutaneous, intramuscular and intraperitoneal inoculation of heartwater infective blood or nymph suspensions was tested in 5 experiments involving a total of 199 sheep. The success rate of subcutaneous ...
  • Heartwater : Past, Present and Future. Workshop (1986 : Kruger National Park, South Africa); Schroder, J.; Bigalke, R.D. (Published by the Government Printer, Pretoria., 1987)
    This paper reviews available literature on the efficacy of acaricides against Amblyomma hebraeum and other tick species, and presents information on tests done with registered chemicals in the laboratory. Little published ...