dc.contributor.author |
Bellieny-Rabelo, Daniel
|
|
dc.contributor.author |
Pretorius, Willem J.S.
|
|
dc.contributor.author |
Moleleki, Lucy Novungayo
|
|
dc.date.accessioned |
2022-03-09T05:39:59Z |
|
dc.date.available |
2022-03-09T05:39:59Z |
|
dc.date.issued |
2021-03 |
|
dc.description.abstract |
Two-component systems (TCS) are important types of machinery allowing for efficient signal recognition and transmission in
bacterial cells. The majority of TCSs utilized by bacteria is composed of a sensor histidine kinase (HK) and a cognate response
regulator (RR). In the present study,we report two newly predicted protein domains—both to be included in the next release of the
Pfamdatabase: Response_reg_2 (PF19192) andHEF_HK(PF19191)—in bacteriawhich exhibit high structural similarity, respectively,
with typical domains of RRs and HKs. Additionally, the genes encoding for the novel predicted domains exhibit a 91.6% linkage
observed across 644 genomic regions recovered from 628 different bacterial strains. The remarkable adjacent colocalization between
genes carrying Response_reg_2 and HEF_HK in addition to their conserved structural features, which are highly similar to
those fromwell-known HKs and RRs, raises the possibility of Response_reg_2 and HEF_HK constituting a new TCS in bacteria. The
genomic regions inwhich these predicted two-component systems-like are located additionally exhibit an overrepresented presence
of restriction–modification (R–M) systems especially the type II R–M. Among these, there is a conspicuous presence of C-5 cytosinespecific
DNAmethylaseswhichmay indicate a functional associationwith the newly discovered domains. The solid presence of R–M
systems and the presence of theGHKL family domain HATPase_c_3 across most of the HEF_HK-containing genes are also indicative
that these genes are evolutionarily related to the paraMORC family of ATPases. |
en_ZA |
dc.description.department |
Biochemistry |
en_ZA |
dc.description.department |
Forestry and Agricultural Biotechnology Institute (FABI) |
en_ZA |
dc.description.department |
Genetics |
en_ZA |
dc.description.department |
Microbiology and Plant Pathology |
en_ZA |
dc.description.librarian |
am2022 |
en_ZA |
dc.description.sponsorship |
The National Research Foundation (NRF), the Research Technology Fund and the University of Pretoria Postdoctoral Fellowship. |
en_ZA |
dc.description.uri |
http://gbe.oxfordjournals.org |
en_ZA |
dc.identifier.citation |
Bellieny-Rabelo, D., Pretorius, W.J.S. & Moleleki, L.N. 2021, 'Novel two-component system-like elements reveal functional domains associated with restriction-modification systems and paraMORC ATPases in bacteria', Genome Biology and Evolution, vol. 13, no. 3, pp. 1-15. |
en_ZA |
dc.identifier.issn |
1759-6653 |
|
dc.identifier.other |
10.1093/gbe/evab024 |
|
dc.identifier.uri |
http://hdl.handle.net/2263/84391 |
|
dc.language.iso |
en |
en_ZA |
dc.publisher |
Oxford University Press |
en_ZA |
dc.rights |
© The Author(s) 2021.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License. |
en_ZA |
dc.subject |
MORC |
en_ZA |
dc.subject |
GHKL ATPases |
en_ZA |
dc.subject |
DNA modification methylases |
en_ZA |
dc.subject |
DNA restriction–modification enzymes |
en_ZA |
dc.subject |
Histidine kinase |
en_ZA |
dc.subject |
Two-component system (TCS) |
en_ZA |
dc.subject |
Deoxyribonucleic acid (DNA) |
en_ZA |
dc.title |
Novel two-component system-like elements reveal functional domains associated with restriction-modification systems and paraMORC ATPases in bacteria |
en_ZA |
dc.type |
Article |
en_ZA |