dc.contributor.author |
MacDonald, Jaime Claire
|
|
dc.contributor.author |
Groome, Michelle J.
|
|
dc.contributor.author |
Janet, Janet
|
|
dc.contributor.author |
Page, Nicola Anne
|
|
dc.date.accessioned |
2020-12-28T06:11:02Z |
|
dc.date.available |
2020-12-28T06:11:02Z |
|
dc.date.issued |
2020-09 |
|
dc.description |
Supplementary Material : File: Final DBS Cohort Results_7.9.2020. |
en_ZA |
dc.description.abstract |
Gastroenteritis is a preventable cause of morbidity and mortality worldwide. Rotavirus
vaccination has significantly reduced the disease burden, but the sub-optimal vaccine efficacy observed
in low-income regions needs improvement. Rotavirus VP4 ‘spike’ proteins interact with FUT2-defined,
human histo-blood group antigens on mucosal surfaces, potentially influencing strain circulation
and the efficacy of P[8]-based rotavirus vaccines. Secretor status was investigated in 500 children
<5 years-old hospitalised with diarrhoea, including 250 previously genotyped rotavirus-positive cases
(P[8] = 124, P[4] = 86, and P[6] = 40), and 250 rotavirus-negative controls. Secretor status genotyping
detected the globally prevalent G428A single nucleotide polymorphism (SNP) and was confirmed by
Sanger sequencing in 10% of participants. The proportions of secretors in rotavirus-positive cases
(74%) were significantly higher than in the rotavirus-negative controls (58%; p < 0.001). The rotavirus
genotypes P[8] and P[4] were observed at significantly higher proportions in secretors (78%) than
in non-secretors (22%), contrasting with P[6] genotypes with similar proportions amongst secretors
(53%) and non-secretors (47%; p = 0.001). This suggests that rotavirus interacts with secretors
and non-secretors in a VP4 strain-specific manner; thus, secretor status may partially influence
rotavirus VP4 wild-type circulation and P[8] rotavirus vaccine efficacy. The study detected a mutation
(rs1800025) ~50 bp downstream of the G428A SNP that would overestimate non-secretors in African
populations when using the TaqMan®SNP Genotyping Assay. |
en_ZA |
dc.description.department |
Medical Virology |
en_ZA |
dc.description.librarian |
pm2020 |
en_ZA |
dc.description.sponsorship |
Rotavirus Surveillance |
en_ZA |
dc.description.sponsorship |
Poliomyelitis Research Foundation |
en_ZA |
dc.description.uri |
http://www.mdpi.com/journal/pathogens |
en_ZA |
dc.identifier.citation |
MacDonald, J., Groome, M.J., Mans, J. et al. 2020, 'FUT2 secretor status influences susceptibility to VP4 strain-specific rotavirus infections in South
African children', Pathogens, vol. 9, no. 10, art. 795, pp. 1-9. |
en_ZA |
dc.identifier.issn |
2076-0817 (online) |
|
dc.identifier.other |
10.3390/pathogens9100795 |
|
dc.identifier.uri |
http://hdl.handle.net/2263/77503 |
|
dc.language.iso |
en |
en_ZA |
dc.publisher |
MDPI |
en_ZA |
dc.rights |
© 2020 by the authors. Licensee: MDPI, Basel, Switzerland. This article is an open access
article distributed under the terms and conditions of the Creative Commons Attribution
(CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
en_ZA |
dc.subject |
Rotavirus |
en_ZA |
dc.subject |
Secretor status |
en_ZA |
dc.subject |
Histo-blood group antigens |
en_ZA |
dc.subject |
VP4 genotypes |
en_ZA |
dc.subject |
FUT2 |
en_ZA |
dc.subject |
Susceptibility |
en_ZA |
dc.subject |
Vaccines |
en_ZA |
dc.title |
FUT2 secretor status influences susceptibility to VP4 strain-specific rotavirus infections in South African children |
en_ZA |
dc.type |
Article |
en_ZA |