Additional file 1: Table S1. Detection rates of immune factors in samples
with and without sub-clinical mastitis, by HIV group. This table compares
the detection rates of immune factors measured in mature breast milk
samples with and without sub-clinical mastitis, separately for samples
from HIV-infected and HIV-uninfected women. There were no detection
rate differences between samples with and without SCM in samples from
HIV-infected women. In comparison, in samples from HIV-uninfected
women, interferon-α, interferon-γ, interleukin-4, α-defensin, Tumor Necrosis
Factor-α and interleukin-6 were more frequently detected in samples
with SCM compared to samples without SCM.
Additional file 2: Table S2. Multivariate models assessing the effect
of HIV on immunologic factor concentration in samples without SCM.
This table indicates the adjusted regression coefficients and associated
p-values of multivariate mixed linear models assessing the effect of HIV
infection on each breast milk soluble immunologic factor concentration,
in samples without sub-clinical mastitis, adjusted on child age at the time
of sampling. HIV infection was associated with an increase of monokine
induced by gamma interferon, inflammatory protein-10, C-reactive protein and ß2-microglobuline, and a decrease of receptor antagonist of
interleukin 1ß and soluble CD14.
Additional file 3: Table S3. Correlations between breast milk immune
factor concentration, HIV plasma parameters and breast milk HIV RNA. This
table indicates the Spearman’s non parametric correlations and associated
p-values between breast milk soluble immunologic factor concentration
and plasma HIV viral load (approximately 6 months after delivery), plasma
CD4 count (approximately 6 months after delivery) and breast milk HIV
RNA (at the time of breast milk sampling), in samples from HIV-infected
women only. There are only a few weak correlations (maximum |ρ| = 0.44).
Additional file 4: Table S4. Breast milk immune factor comparisons
between samples with sub-clinical mastitis from HIV-uninfected and
HIV-infected women. This table compares the concentration of immune
factors measured in mature breast milk samples with sub-clinical mastitis,
between samples from HIV-infected and HIV-uninfected women. There
were no statistically significant concentration differences in samples with
sub-clinical mastitis between HIV-infected and HIV-uninfected women.
Additional file 5: Table S5. Multivariate models assessing the effect
of SCM on immunologic factor concentration, by HIV group. This table
indicates the adjusted regression coefficients and associated p-values of
multivariate mixed linear models assessing the effect of sub-clinical mastitis
on each breast milk soluble factor concentration, adjusted on child
age at the time of sampling, separately for samples from HIV-infected and
HIV-uninfected women. Sub-clinical mastitis was associated with and
increase of 9/13 immune factors analyzed in samples from HIV-uninfected
women compared to 7/17 immune factors analyzed in samples from
HIV-infected women.