dc.contributor.author |
Bester, L.A.
|
|
dc.contributor.author |
Phulukdaree, Alisa
|
|
dc.contributor.author |
Khan, N.
|
|
dc.contributor.author |
Essack, S.Y.
|
|
dc.date.accessioned |
2017-02-21T06:24:10Z |
|
dc.date.available |
2017-02-21T06:24:10Z |
|
dc.date.issued |
2016-06 |
|
dc.description.abstract |
This study compared phenotypic and genotypic identification methods of Campylobacter spp. against the polymerase chain reaction
(PCR) in terms of sensitivity, specificity, positive-predictive value and negative-predictive value. Thermophilic Campylobacter
isolates were identified using conventional biochemical tests, specifically hippurate hydrolysis, matrix assisted laser desorption
ionization- time of flight (MALDI-ToF) mass spectrometry and PCR with primers unique to C. jejuni and C. coli. MALDI-ToF was
shown to be superior to biochemical tests for identification of C. coli but equivalent to biochemical tests for C. jejuni. |
en_ZA |
dc.description.department |
Physiology |
en_ZA |
dc.description.librarian |
am2017 |
en_ZA |
dc.description.sponsorship |
The National Research Foundation
Thuthuka: Researchers in Training Programme Ref.
TTK2007040500009 |
en_ZA |
dc.description.uri |
http://www.smltsa.org.za |
en_ZA |
dc.identifier.citation |
Bester, LA, Phulukdaree, A, Khan, N & Essack 2016, 'Efficacy of phenotypic, PCR and MALDI -ToF identification methods for Campylobacter spp.', Medical Technology SA, vol. 30, no. 1, pp. 49-51. |
en_ZA |
dc.identifier.issn |
1011-5528 |
|
dc.identifier.uri |
http://hdl.handle.net/2263/59124 |
|
dc.language.iso |
en |
en_ZA |
dc.publisher |
Society of Medical Laboratory Technologists of South Africa |
en_ZA |
dc.rights |
Society of Medical Laboratory Technologists of South Africa |
en_ZA |
dc.subject |
Polymerase chain reaction (PCR) |
en_ZA |
dc.subject |
Matrix assisted laser desorption ionization-time of flight (MALDI-ToF) |
en_ZA |
dc.subject |
Campylobacter spp. |
en_ZA |
dc.title |
Efficacy of phenotypic, PCR and MALDI -ToF identification methods for Campylobacter spp. |
en_ZA |
dc.type |
Article |
en_ZA |