Efficacy of phenotypic, PCR and MALDI -ToF identification methods for Campylobacter spp.

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Authors

Bester, L.A.
Phulukdaree, Alisa
Khan, N.
Essack, S.Y.

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Society of Medical Laboratory Technologists of South Africa

Abstract

This study compared phenotypic and genotypic identification methods of Campylobacter spp. against the polymerase chain reaction (PCR) in terms of sensitivity, specificity, positive-predictive value and negative-predictive value. Thermophilic Campylobacter isolates were identified using conventional biochemical tests, specifically hippurate hydrolysis, matrix assisted laser desorption ionization- time of flight (MALDI-ToF) mass spectrometry and PCR with primers unique to C. jejuni and C. coli. MALDI-ToF was shown to be superior to biochemical tests for identification of C. coli but equivalent to biochemical tests for C. jejuni.

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Keywords

Polymerase chain reaction (PCR), Matrix assisted laser desorption ionization-time of flight (MALDI-ToF), Campylobacter spp.

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Citation

Bester, LA, Phulukdaree, A, Khan, N & Essack 2016, 'Efficacy of phenotypic, PCR and MALDI -ToF identification methods for Campylobacter spp.', Medical Technology SA, vol. 30, no. 1, pp. 49-51.