Efficacy of phenotypic, PCR and MALDI -ToF identification methods for Campylobacter spp.

dc.contributor.authorBester, L.A.
dc.contributor.authorPhulukdaree, Alisa
dc.contributor.authorKhan, N.
dc.contributor.authorEssack, S.Y.
dc.date.accessioned2017-02-21T06:24:10Z
dc.date.available2017-02-21T06:24:10Z
dc.date.issued2016-06
dc.description.abstractThis study compared phenotypic and genotypic identification methods of Campylobacter spp. against the polymerase chain reaction (PCR) in terms of sensitivity, specificity, positive-predictive value and negative-predictive value. Thermophilic Campylobacter isolates were identified using conventional biochemical tests, specifically hippurate hydrolysis, matrix assisted laser desorption ionization- time of flight (MALDI-ToF) mass spectrometry and PCR with primers unique to C. jejuni and C. coli. MALDI-ToF was shown to be superior to biochemical tests for identification of C. coli but equivalent to biochemical tests for C. jejuni.en_ZA
dc.description.departmentPhysiologyen_ZA
dc.description.librarianam2017en_ZA
dc.description.sponsorshipThe National Research Foundation Thuthuka: Researchers in Training Programme Ref. TTK2007040500009en_ZA
dc.description.urihttp://www.smltsa.org.zaen_ZA
dc.identifier.citationBester, LA, Phulukdaree, A, Khan, N & Essack 2016, 'Efficacy of phenotypic, PCR and MALDI -ToF identification methods for Campylobacter spp.', Medical Technology SA, vol. 30, no. 1, pp. 49-51.en_ZA
dc.identifier.issn1011-5528
dc.identifier.urihttp://hdl.handle.net/2263/59124
dc.language.isoenen_ZA
dc.publisherSociety of Medical Laboratory Technologists of South Africaen_ZA
dc.rightsSociety of Medical Laboratory Technologists of South Africaen_ZA
dc.subjectPolymerase chain reaction (PCR)en_ZA
dc.subjectMatrix assisted laser desorption ionization-time of flight (MALDI-ToF)en_ZA
dc.subjectCampylobacter spp.en_ZA
dc.titleEfficacy of phenotypic, PCR and MALDI -ToF identification methods for Campylobacter spp.en_ZA
dc.typeArticleen_ZA

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