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| dc.contributor.author | Visser, Elizabeth S.
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| dc.contributor.author | Ambrosio, R.E.
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| dc.contributor.editor | Bigalke, R.D. | |
| dc.date.accessioned | 2014-11-17T08:54:18Z | |
| dc.date.available | 2014-11-17T08:54:18Z | |
| dc.date.created | 2014 | |
| dc.date.issued | 1987 | |
| dc.description | The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format. | en_US |
| dc.description.abstract | Anaplasmosis can be diagnosed either by immunological techniques or by direct microscopic examination of blood smears. Both methods are time-consuming and labour intensive. The use of DNA probes in an hybridization assay may simplify the diagnosis of anaplasmosis in cattle and sheep. A genomic DNA library of Anaplasma centrale was constructed in an expression vector and screened to detect clones containing A. centrale DNA. Four probes which hybridized to A. centrale and Anaplasma marginale DNA were isolated. One of these (AC-1) hybridized only to A. centrale DNA, whereas AC-2, AC-3 and AC-4 could detect DNA from both A. centrale and A. marginale. Probes AC-1 and AC-2 could detect 127 ng and 8 ng DNA respectively, while AC-3 and AC-4 detected 64 ng A. centrale DNA. | en_US |
| dc.description.librarian | lmchunu2014 | en_US |
| dc.description.librarian | mn2014 | |
| dc.identifier.citation | Visser, ES & Ambrosio, RE 1987, 'DNA probes for the detection of Anaplasma centrale and Anaplasma marginale’, Onderstepoort Journal of Veterinary Research, vol. 54, no. 4, pp. 623-627. | en_US |
| dc.identifier.issn | 0330-2465 | |
| dc.identifier.uri | http://hdl.handle.net/2263/42599 | |
| dc.language.iso | en | en_US |
| dc.publisher | Published by the Government Printer, Pretoria. | en_US |
| dc.rights | ©ARC - Onderstepoort and Faculty of Veterinary Science, University of Pretoria (original). ©University of Pretoria. Dept. of Library Services (digital). | en_US |
| dc.subject | Veterinary medicine | en_US |
| dc.subject.lcsh | Veterinary medicine -- South Africa | |
| dc.title | DNA probes for the detection of Anaplasma centrale and Anaplasma marginale | en_US |
| dc.type | Article | en_US |
