DNA probes for the detection of Anaplasma centrale and Anaplasma marginale
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Date
Authors
Visser, Elizabeth S.
Ambrosio, R.E.
Journal Title
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Volume Title
Publisher
Published by the Government Printer, Pretoria.
Abstract
Anaplasmosis can be diagnosed either by immunological techniques or by direct microscopic examination of blood smears. Both methods are time-consuming and labour intensive. The use of DNA probes in an hybridization assay may simplify the diagnosis of anaplasmosis in cattle and sheep. A genomic DNA library of Anaplasma centrale was constructed in an expression vector and screened to detect clones containing A. centrale DNA. Four probes which hybridized to A. centrale and Anaplasma marginale DNA were isolated. One of these (AC-1) hybridized only to A. centrale DNA, whereas AC-2, AC-3 and AC-4 could detect DNA from both A. centrale and A. marginale. Probes AC-1 and AC-2 could detect 127 ng and 8 ng DNA respectively, while AC-3 and AC-4 detected 64 ng A. centrale DNA.
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Keywords
Veterinary medicine
Sustainable Development Goals
Citation
Visser, ES & Ambrosio, RE 1987, 'DNA probes for the detection of Anaplasma centrale and Anaplasma marginale’, Onderstepoort Journal of Veterinary Research, vol. 54, no. 4, pp. 623-627.