Additional file 1: Growth curve and gas composition during the
cultivation of P. thermoglucosidasius), DSM 2542T (A), DSM 2543 (B) and
DSM 6285 (C), DSM 21625 (D). All strains were cultivated in quadruplicate
in mLB medium with an initial gas atmosphere consisting of 50% CO and
50% air for 84 h.
Additional file 2: Genomic relatedness among the four compared P.
thermoglucosidasius strains. Calculation of the digital DNA-DNA
hybridization (GGDC) [19] and OrthoANI [20] values for each paired combination
of strains. The GGDC are the bottom and the OrthoANI the top
values.
Additional file 3: Annotations of the protein families shared and unique
among the compared P. thermoglucosidasius strains. The protein family
datasets which are shared between different combinations of the four
compared strains or unique to a particular strain were functionally
annotated by RAST [36], comparison against the Conserved Domain
Database [37] and classification according to their COG function using
EggNOG mapper [36]. The proportions (%) of proteins (unique to strains
or shared among different combinations of strains) belonging to each
COG are graphically presented.
Additional file 4: SNPs occurring in the CODH-NiFe group 4a locus
genes of the compared P. thermoglucosidasius strains. The number of
SNPs occurring in the individual CODH-NiFe group 4a genes of particular
strains are indicated. The number in brackets indicates the number of
non-synonymous amino acid substitutions observed in the amino acid
sequence alignments for each individual gene.