Browsing 2002 Onderstepoort Journal of Veterinary Research, Volume 69, 2002 by Issue Date

Browsing 2002 Onderstepoort Journal of Veterinary Research, Volume 69, 2002 by Issue Date

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  • The prevalence and economic importance of bovine fasciolosis in Kenya - an analysis of abattoir data 
    Kithuka, J.M.; Maingi, N.; Njeruh, F.M.; Ombui, J.N.; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    A retrospective study covering a period of 10 years (1990-1999) was carried out using post mortem meat inspection records at the Veterinary Department Headquarters at Kabete to determine the prevalence and economic importance ...
  • Multiplication of the V4 strain of Newcastle disease virus in Madin Derby bovine kidney cells 
    Sahle, M.; Burgess, W.G.; Kidanemariam, Awoke; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    This study describes a reproducible cell culture system that permits the growth and secondary multiplication of the V4 strain of Newcastle disease virus. Allantoic fluid, magnesium chloride and diethylaminoethyl dextran ...
  • Development of solid phase antigen for indirect ELISA for the detection of specific antibody responses to infection with Newcastle disease virus 
    Sahle, M.; Burgess, G.W.; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    A simple and inexpensive method of antigen preparation by ultrafiltration was investigated using the V4 strain of Newcastle disease virus. The antigen designated XM300 was used in an indirect enzyme-linked immunosorbent ...
  • Development of a diagnostic one-tube RT-PCR for the detection of Rift Valley fever virus 
    Nel, Louis Hendrik; Espach, Anel; Romito, M.; Viljoen, G.J.; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    Diagnosis of Rift Valley fever (RVF) is based on serology and virus isolation. The disadvantages of the former include poor sensitivity, high cost, risks associated with using infectious virus as antigen, the lengthy ...
  • Virulence of South African isolates of Haemophilus paragallinarum. Part 3: Experimentally produced NAD-independent isolate 
    Taole, M.; Albertyn, J.; Van Heerden, E.; Bragg, R.R. (Robert Richard); Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    A NAD-dependent isolate 46 (C-3) of Haemophilus paragallinarum, which was previously demonstrated to be of high virulence, was transformed to NAD independence using a plasmid isolated from a naturally occurring NAD-independent ...
  • Seasonal abundance of four Culicoides spp. (Diptera: Ceratopogonidae) at Al-Ahsa oasis, Eastern Province, Saudi Arabia 
    Abu Elzein, E.M.E.; Hilali, M.; Al-Afaleq, A.I.; Mellor, Philip S.; Boorman, J.; Al-Atiya, S.; Al-Naiem, A.; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    This report constitutes the first study of Culicoides spp. and their seasonal abundance at Al-Ahsa, the largest oasis in the Eastern Province of Saudi Arabia. New Jersey light traps were used to collect the midges at Mastock ...
  • The classification of seven serotypes of equine encephalosis virus and the prevalence of homologous antibody in horses in South Africa 
    Howell, P.G.; Bosman, Anna-Mari; Coetzer, Jacobus A.W.; Guthrie, Alan John; Groenewald, Daleen; Visage, Carina W.; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    Selected isolates of equine encephalosis virus were shown to have comparable viral protein profiles and to represent seven distinct serotypes, based on cross-neutralization tests. Serotype-specific virus-neutralizing ...
  • Description of Pelonia africana n. g., n. sp. (Pentastomida: Sebekidae) from the lungs of Pelomedusa subrufa and Pelusios sinuatus (Chelonia) in South Africa 
    Boomker, Jacob Diederik Frederik; Junker, Kerstin; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    The terrapins Pelomedusa subrufa and Pelusios sinuatus taken from a water reservoir in the Northern Province, South Africa, were examined for pentastome infections. Two immature specimens, a patent female and a mature male, ...
  • Non-immune control of trypanosomosis: in vitro oxidative burst of PMA- and trypanosome-stimulated neutrophils of Boran and N'Dama cattle 
    Taiwo, V.O.; Adejinmi, J.O.; Oluwaniyi, J.O.; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    An in vitro assay that measures the generation of superoxide anions (O₂ˉ) was used to assess the level of oxidative burst of phorbol myristate acetate (PMA) - and trypanosome-stimulated neutrophils isolated from healthy ...
  • Subject index 
    Anonymous; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
  • Hepatic and renal concentrations of copper and other trace elements in hippopotami (Hippopotamus amphibius L.) living in and adjacent to the Kafue and Luangwa Rivers in Zambia 
    Mwase, M.; Almli, B.; Sivertsen, T.; Musonda, M.M.; Flaoyen, A.; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    Hepatic and renal concentrations of the elements arsenic, cadmium, cobalt, copper, lead, manganese, mercury, molybdenum, selenium and zinc were studied in samples collected from hippopotami from the Kafue River in the Kafue ...
  • Parasitological prevalence of bovine trypanosomosis in Kindo Koisha district, Wollaita zone, south Ethiopia 
    Kidanemariam, Awoke; Hadgu, K.; Sahle, M.; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    A cross sectional survey to determine the distribution and prevalence of trypanosomosis was conducted in Kindo Koisha district, in the Wollaita zone in southern Ethiopia. A total of 1008 adult cattle was examined at eight ...
  • Teratogenicity of a mutagenised Rift Valley fever virus (MVP 12) in sheep 
    Hunter, P.; Erasmus, B.J.; Vorster, J.H. (Jan Harm); Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    A 5-fluorouracil mutagenised Rift Valley fever virus strain, which was shown to be attenuated and immunogenic in cattle and sheep, was evaluated for its ability to cause teratogenic effects in pregnant sheep. A group of ...
  • Book review 
    Anonymous; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
  • Animal-level risk factors for Trypanosoma evansi infection in camels in eastern and central parts of Kenya 
    Ngaira, J.M.; Bett, B.; Karanja, S.M.; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    Point prevalences and animal-level risk factors for Trypanosoma evansi infection were investigated in a cross-sectional study that involved 2227 camels from eastern and central parts of Kenya. The screening tests used were ...
  • Hosts, seasonal occurrence and life cycle of Rhipicentor nuttalli (Acari: Ixodidae) 
    Horak, Ivan Gerard; Fourie, L.J.; Kok, D.J.; Van Zyl, W.; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    There are only two species worldwide within the genus Rhipicentor, namely Rhipicentor bicornis and Rhipicentor nuttalli, and both occur only in Africa. Rhipicentor nuttalli has a widespread distribution in South Africa and ...
  • Virulence of South African isolates of Haemophilus paragallinarum. Part 1: NAD-dependent field isolates 
    Bragg, R.R. (Robert Richard); Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    The virulence of four South African field isolates of NAD-dependent Haemophilus paragallinarum, representing the four serovars known to occur in that country, was investigated. During this study an alternative challenge ...
  • An overview of the eradication of Brucella melitensis from KwaZulu-Natal 
    Emslie, Forbes Richard; Nel, J.R.; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    Brucella melitensis is a Gram-negative bacterium whose primary hosts are goats and sheep. Like the other Brucella spp., with the exception of Brucella ovis, it is not particularly host specific as it is pathogenic for a ...
  • Validation of an indirect enzyme-linked immunosorbent assay for the detection of antibody against Brucella abortus in cattle sera using an automated ELISA workstation 
    Paweska, Janusz Tadeusz; Prozesky, Leon; Potts, A.D.; Harris, H.J.; Smith, S.J.; Viljoen, Gerrit Johannes; Dungu, B.; Brett, O.L.; Bubb, M.; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    An automated indirect enzyme-linked immunosorbent assay (I-ELISA) for the serological diagnosis of bovine brucellosis was developed and validated in-house. A total of 4803 cattle sera from South Africa ( n=3 643), Canada ...
  • In vitro isolation of equine piroplasms derived from Cape Mountain zebra (Equus zebra zebra) in South Africa 
    Zweygarth, Erich; Meyer, P.; Lopez-Rebollar, Laura M.; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    Twenty blood samples of zebras ( Equus zebra zebra) from the Karoo National Park and the Bontebok National Park in South Africa, all seropositive for Theileria equi, were subjected to in vitro culture to identify carrier ...