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The soil microbiomes of forest ecosystems in Kenya : their diversity and environmental drivers

dc.contributor.authorOnyango, Lorine Akinyi
dc.contributor.authorNgonga, Florence Atieno
dc.contributor.authorKaranja, Edward Nderitu
dc.contributor.authorKuja, Josiah Ochieng’
dc.contributor.authorBoga, Hamadi Iddi
dc.contributor.authorCowan, Don A.
dc.contributor.authorMwangi, Kennedy Wanjau
dc.contributor.authorMaghenda, Marianne Wughanga
dc.contributor.authorMarinho Lebre, Pedro Bixirao Neto
dc.contributor.authorKambura, Anne Kelly
dc.date.accessioned2024-02-23T13:17:24Z
dc.date.available2024-02-23T13:17:24Z
dc.date.issued2023-05-02
dc.descriptionDATA AVAILABILITY : The demultiplexed high-quality sequence reads has been deposited in the National Centre for Biotechnology Information (NCBI) Sequence Read Archive (SRA), as Bio Project ID: PRJNA851255 and study accession numbers available for download at http://www.ncbi.nlm.nih.gov/biopr oject/851255. This Whole Genome Shotgun project has been deposited at DDBJ/EMBL/GenBank under the Bioproject PRJNA291812. The metadata, soil chemistry data, input files for Qiime and R analysis scripts were deposited at https://zenodo.org/ and a DOI-10.5281/zenodo.7827433 available using the link; https://doi.org/10.5281/zenodo.78274 32.en_US
dc.description.abstractSoil microbiomes in forest ecosystems act as both nutrient sources and sinks through a range of processes including organic matter decomposition, nutrient cycling, and humic compound incorporation into the soil. Most forest soil microbial diversity studies have been performed in the northern hemisphere, and very little has been done in forests within African continent. This study examined the composition, diversity and distribution of prokaryotes in Kenyan forests top soils using amplicon sequencing of V4-V5 hypervariable region of the 16S rRNA gene. Additionally, soil physicochemical characteristics were measured to identify abiotic drivers of prokaryotic distribution. Different forest soils were found to have statistically distinct microbiome compositions, with Proteobacteria and Crenarchaeota taxa being the most differentially abundant across regions within bacterial and archaeal phyla, respectively. Key bacterial community drivers included pH, Ca, K, Fe, and total N while archaeal diversity was shaped by Na, pH, Ca, total P and total N. To contextualize the prokaryote diversity of Kenyan forest soils on a global scale, the sample set was compared to amplicon data obtained from forest biomes across the globe; displaying them to harbor distinct microbiomes with an over-representation of uncultured taxa such as TK-10 and Ellin6067 genera.en_US
dc.description.departmentBiochemistryen_US
dc.description.departmentGeneticsen_US
dc.description.departmentMicrobiology and Plant Pathologyen_US
dc.description.librarianam2024en_US
dc.description.sdgSDG-15:Life on landen_US
dc.description.sponsorshipUSAID, The Oppenheimer Foundation and the University of Pretoria.en_US
dc.description.urihttps://www.nature.com/srepen_US
dc.identifier.citationOnyango, L.A., Ngonga, F.A., Karanja, E.N. et al. 2023, 'The soil microbiomes of forest ecosystems in Kenya : their diversity and environmental drivers', Scientific Reports, vol. 13, art. 7156, pp. 1-14. https://DOI.org/10.1038/s41598-023-33993-4.en_US
dc.identifier.issn2045-2322 (online)
dc.identifier.other10.1038/s41598-023-33993-4
dc.identifier.urihttp://hdl.handle.net/2263/94920
dc.language.isoenen_US
dc.publisherNature Reseachen_US
dc.rights© The Author(s) 2023. This article is licensed under a Creative Commons Attribution 4.0 International License.en_US
dc.subjectSoil microbiomesen_US
dc.subjectCompositionen_US
dc.subjectDiversityen_US
dc.subjectAfrican continenten_US
dc.subjectSDG-15: Life on landen_US
dc.titleThe soil microbiomes of forest ecosystems in Kenya : their diversity and environmental driversen_US
dc.typeArticleen_US

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