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dc.contributor.author | Van Heerden, Alishia![]() |
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dc.contributor.author | Pham, Nam Q.![]() |
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dc.contributor.author | Wingfield, Brenda D.![]() |
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dc.contributor.author | Wingfield, Michael J.![]() |
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dc.contributor.author | Muro Abad, Jupiter I.![]() |
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dc.contributor.author | Duran, Alvaro![]() |
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dc.contributor.author | Wilken, Pieter Markus![]() |
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dc.date.accessioned | 2024-08-28T05:29:48Z | |
dc.date.available | 2024-08-28T05:29:48Z | |
dc.date.issued | 2024 | |
dc.description.abstract | Eucalyptus scab and shoot malformation caused by Elsinoë necatrix is an emerging disease and a serious threat to the global commercial forestry industry. The disease was first discovered in North Sumatra, Indonesia, and now requires a simple and effective method for early pathogen detection. In this study, a rapid and sensitive loop-mediated isothermal amplification (LAMP) assay was developed for E. necatrix. A unique region in a secondary metabolite gene cluster was used as a target for the assay. To test robustness of the assay, LAMP was verified in 15 strains of E. necatrix. A specificity test against 23 closely related Elsinoë species and three fungal species commonly isolated on Eucalyptus showed that the LAMP assay exclusively identified E. necatrix isolates. The assay had a high level of sensitivity, able to detect 0.01 ng (approximately 400 target copies) of pure E. necatrix DNA. Furthermore, using a simple DNA extraction method, it was possible to use this assay to detect E. necatrix in infected Eucalyptus leaves. | en_US |
dc.description.department | Biochemistry | en_US |
dc.description.department | Forestry and Agricultural Biotechnology Institute (FABI) | en_US |
dc.description.department | Genetics | en_US |
dc.description.department | Microbiology and Plant Pathology | en_US |
dc.description.librarian | hj2024 | en_US |
dc.description.sdg | SDG-15:Life on land | en_US |
dc.description.sponsorship | National Research Foundation, South Africa. | en_US |
dc.description.uri | https://apsjournals.apsnet.org/loi/pdis | en_US |
dc.identifier.citation | Van Heerden, A., Pham, N.Q., Wingfield, B.D. et al. 2024, 'LAMP assay to detect Elsinoë necatrix, an important Eucalyptus shoot and leaf pathogen', Plant Disease, doi : 10.1094/PDIS-01-24-0086-RE. | en_US |
dc.identifier.issn | 0191-2917 (print) | |
dc.identifier.issn | 1943-7692 (online) | |
dc.identifier.other | 10.1094/PDIS-01-24-0086-RE | |
dc.identifier.uri | http://hdl.handle.net/2263/97896 | |
dc.language.iso | en | en_US |
dc.publisher | American Phytopathological Society | en_US |
dc.rights | © 2024 The American Phytopathological Society. | en_US |
dc.subject | Eucalyptus scab | en_US |
dc.subject | Shoot malformation | en_US |
dc.subject | Elsinoë necatrix | en_US |
dc.subject | Forestry | en_US |
dc.subject | Loop-mediated isothermal amplification (LAMP) | en_US |
dc.subject | Scab disease | en_US |
dc.subject | Diagnostic assay | en_US |
dc.subject | LAMP assay | en_US |
dc.subject | Plantation forestry | en_US |
dc.subject | SDG-15: Life on land | en_US |
dc.title | LAMP assay to detect Elsinoë necatrix, an important Eucalyptus shoot and leaf pathogen | en_US |
dc.type | Postprint Article | en_US |