Please be advised that the site will be down for maintenance on Sunday, September 1, 2024, from 08:00 to 18:00, and again on Monday, September 2, 2024, from 08:00 to 09:00. We apologize for any inconvenience this may cause.
dc.contributor.author | Midgley, Kayla Alexis![]() |
|
dc.contributor.author | Van den Berg, Noelani![]() |
|
dc.contributor.author | Backer, Robert![]() |
|
dc.contributor.author | Swart, Velushka![]() |
|
dc.date.accessioned | 2024-08-07T13:12:11Z | |
dc.date.available | 2024-08-07T13:12:11Z | |
dc.date.issued | 2024-05 | |
dc.description | DATA AVAILABILITY STATEMENT: Data generated or analysed during this study are included in this published article and its supplementary information files. Sequences used in this study are available on Genbank (NCBI) accession numbers OR501732 - OR501777. All P. cinnamomi cultures are available in the ARP culture collection at the University of Pretoria, South Africa. | en_US |
dc.description.abstract | The oomycete Phytophthora cinnamomi is a devastating plant pathogen with a notably broad host range. It is the causal agent of Phytophthora root rot (PRR), arguably the most economically important yield-limiting disease in Persea americana (avocado). Despite this, our understanding of the mechanisms P. cinnamomi employs to infect and successfully colonize avocado remains limited, particularly regarding the pathogen’s ability to maintain its biotrophic and necrotrophic lifestyles during infection. The pathogen utilises a large repertoire of effector proteins which function in facilitating and establishing disease in susceptible host plants. Crinkling and necrosis effectors (CRN/Crinklers) are suspected to manipulate cell death to aid in maintenance of the pathogens biotrophic and necrotrophic lifestyles during different stages of infection. The current study identified 25 P. cinnamomi CRN effectors from the GKB4 genome using an HMM profile and assigned putative function to them as either cell death inducers or suppressors. Function was assigned to 10 PcinCRNs by analysing their RNA-seq expression profiles, relatedness to other functionally characterised Phytophthora CRNs and tertiary protein predictions. The full-length coding sequences for these PcinCRNs were confirmed by Sanger sequencing, six of which were found to have two divergent alleles. The presence of alleles indicates that the proteins encoded may perform contradicting functions in cell death manipulation, or function in different host plant species. Overall, this study provides a foundation for future research on P. cinnamomi infection and cell death manipulation mechanisms. | en_US |
dc.description.department | Biochemistry | en_US |
dc.description.department | Forestry and Agricultural Biotechnology Institute (FABI) | en_US |
dc.description.department | Genetics | en_US |
dc.description.department | Microbiology and Plant Pathology | en_US |
dc.description.sdg | SDG-15:Life on land | en_US |
dc.description.sponsorship | The Hans Merensky Foundation (HMF) through a grant awarded to the Hans Merensky Chair in Avocado Research at the University of Pretoria. | en_US |
dc.description.uri | https://bmcgeriatr.biomedcentral.com/ | en_US |
dc.identifier.citation | Midgley, K.A., van den Berg, N., Backer, R. et al. Identification of Phytophthora cinnamomi CRN effectors and their roles in manipulating cell death during Persea americana infection. BMC Genomics 25, 435 (2024). https://doi.org/10.1186/s12864-024-10358-3. | en_US |
dc.identifier.issn | 1471-2318 (online) | |
dc.identifier.other | 10.1186/s12864-024-10358-3 | |
dc.identifier.uri | http://hdl.handle.net/2263/97502 | |
dc.language.iso | en | en_US |
dc.publisher | BMC | en_US |
dc.rights | © The Author(s) 2024. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License. | en_US |
dc.subject | Plant-pathogen interactions | en_US |
dc.subject | Crinkler effectors | en_US |
dc.subject | Molecular characterization | en_US |
dc.subject | In silico | en_US |
dc.subject | Protein prediction | en_US |
dc.subject | Alleles | en_US |
dc.subject | Phytophthora | en_US |
dc.subject | Hemibiotroph | en_US |
dc.subject | SDG-15: Life on land | en_US |
dc.title | Identification of Phytophthora cinnamomi CRN effectors and their roles in manipulating cell death during Persea americana infection | en_US |
dc.type | Article | en_US |