dc.contributor.author |
Singh, Ashmika
|
|
dc.contributor.author |
Patel, Nashrin F.
|
|
dc.contributor.author |
Allam, Mushal
|
|
dc.contributor.author |
Chan, Wai Yin
|
|
dc.contributor.author |
Mohale, Thabo
|
|
dc.contributor.author |
Ismail, Arshad
|
|
dc.contributor.author |
Oliver, Shune V.
|
|
dc.date.accessioned |
2023-08-11T10:51:18Z |
|
dc.date.available |
2023-08-11T10:51:18Z |
|
dc.date.issued |
2022-12-16 |
|
dc.description |
DATA AVAILABILITY STATEMENT : All data associated with the manuscript are found in this publication. |
en_US |
dc.description |
SUPPLEMENTARY MATERIAL : FIGURE S1: Bar plot of all specimens. FIGURE S2: rarefaction curves of larval specimens. FIGURE S3: rarefaction curves of adult specimens. FIGURE S4: Differential abundance in larvae. TABLE S1: Operational Taxonomic Unit (OTU) table of sequencing data; TABLE S2: Summary of metagenomic data as per the Mosquito Microbiome Consortium. TABLE S3: Primers used in this study. TABLE S4: Deltamethrin lethal times in the MAFUS strain. |
en_US |
dc.description.abstract |
Anopheles merus can breed in a range of saltwater concentrations. The consequences of this
ability on the life history of adult An. merus are poorly understood. This study examined the effects
of exposure to 0, 2.1875, 4.375, 8.75, and 17.5 g/L of sodium chloride on An. merus. The effects on
larval development, adult longevity, fertility, and fecundity, as well as deltamethrin tolerance were
examined. The effect of larval salt exposure on the expression of defensin-1 in adults was examined by
quantitative Real-Time PCR. Finally, the effect of the larval salt concentration on microbial dynamics
was assessed by 16S Next Generation Sequencing. High concentrations of saltwater increased larval
development time and number of eggs laid, as well as deltamethrin tolerance. Larval exposure to
salt also reduced the expression of defensin-1. The exposure also had a significant effect on microbial
diversity in larvae and adults. The diversity of larvae decreased once adults emerged. Salt-tolerant
bacterial genera predominated in larvae but were absent in adults. High salt concentrations resulted
in greater abundance of Plasmodium-protective genera in adults. Although this study was conducted
on a laboratory strain of An. merus, these data suggest that osmoregulation has a significant effect on
the life history of the species with potential epidemiological consequences. |
en_US |
dc.description.department |
Biochemistry |
en_US |
dc.description.department |
Forestry and Agricultural Biotechnology Institute (FABI) |
en_US |
dc.description.department |
Genetics |
en_US |
dc.description.department |
Microbiology and Plant Pathology |
en_US |
dc.description.librarian |
am2023 |
en_US |
dc.description.sponsorship |
The National Research Foundation of South Africa and a Female Academic Fellowship (NPC) grant. The APC was funded by the National Health Laboratory Services Research Trust. |
en_US |
dc.description.uri |
https://www.mdpi.com/journal/insects |
en_US |
dc.identifier.citation |
Singh, A.; Patel, N.F.;
Allam, M.; Chan,W.-Y.; Mohale, T.;
Ismail, A.; Oliver, S.V. Marked Effects
of Larval Salt Exposure on the Life
History and Gut Microbiota of the
Malaria Vector Anopheles merus
(Diptera: Culicidae). Insects 2022, 13,
1165. https://DOI.org/10.3390/insects13121165. |
en_US |
dc.identifier.issn |
2075-4450 (online) |
|
dc.identifier.other |
10.3390/ insects13121165 |
|
dc.identifier.uri |
http://hdl.handle.net/2263/91888 |
|
dc.language.iso |
en |
en_US |
dc.publisher |
MDPI |
en_US |
dc.rights |
© 2022 by the authors.
Licensee MDPI, Basel, Switzerland.
This article is an open access article
distributed under the terms and
conditions of the Creative Commons
Attribution (CC BY) license. |
en_US |
dc.subject |
Osmoregulation |
en_US |
dc.subject |
Development |
en_US |
dc.subject |
Diversity |
en_US |
dc.subject |
Bacterial abundance |
en_US |
dc.subject |
Anopheles merus |
en_US |
dc.title |
Marked effects of larval salt exposure on the life history and gut microbiota of the malaria vector Anopheles merus (Diptera: Culicidae) |
en_US |
dc.type |
Article |
en_US |