Antifungal activity and mode of action of synthetic peptides derived from the tick OsDef2 defensin
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Date
Authors
Mbuayama, Kabuzi R.
Taute, Helena
Stromstedt, Adam A.
Bester, Megan Jean
Gaspar, Anabella Regina Marques
Journal Title
Journal ISSN
Volume Title
Publisher
Wiley
Abstract
Please read abstract in the article.
Description
Supporting Information: Table S1. Physicochemical properties and antifungal activity of OsDef2 derived peptides.
Figure S1. Effect of sodium azide on the intracellular translocation of 5-FAM penetratin. C. albicans cells were pre-incubated with 5mM NaN3 for 30 min and treated with 5-FAM penetratin. The cells were counterstained with DAPI (blue) and visualized by confocal microscopy. Scale bars are 10 μm.
Figure S2. DAPI stained images of 5-FAM-peptides in C. albicans. Cells were exposed for 50 min to 2.5 μM of 5-FAM-peptides, counterstained with DAPI (blue) and visualized by confocal microscopy. Top and bottom panel show same images at lower and higher magnification, respectively. Short arrow: fragmented nuclear DNA; long arrow: tubular-shaped DNA, in contrast to single and round nucleus observed in untreated cells. Images are representatives of three independent experiments, in duplicate. Scale bars are 10 μm.
Keywords
Antimicrobial peptides, Defensins, Membrane permeabilization, Reactive oxygen species (ROS), Ticks
Sustainable Development Goals
Citation
Mbuayama, K.R., Taute, H., Stromstedt, A.A., Bester, M.J. & Gaspar, A.R.M. Antifungal activity and mode of action of synthetic peptides derived from the tick OsDef2 defensin. Journal of Peptide Science 2022;28(5):e3383.doi:10.1002/psc.3383.