Antifungal activity and mode of action of synthetic peptides derived from the tick OsDef2 defensin

dc.contributor.authorMbuayama, Kabuzi R.
dc.contributor.authorTaute, Helena
dc.contributor.authorStromstedt, Adam A.
dc.contributor.authorBester, Megan Jean
dc.contributor.authorGaspar, Anabella Regina Marques
dc.contributor.emailanabella.gaspar@up.ac.zaen_US
dc.date.accessioned2022-07-25T06:39:59Z
dc.date.issued2022-05
dc.descriptionSupporting Information: Table S1. Physicochemical properties and antifungal activity of OsDef2 derived peptides. Figure S1. Effect of sodium azide on the intracellular translocation of 5-FAM penetratin. C. albicans cells were pre-incubated with 5mM NaN3 for 30 min and treated with 5-FAM penetratin. The cells were counterstained with DAPI (blue) and visualized by confocal microscopy. Scale bars are 10 μm. Figure S2. DAPI stained images of 5-FAM-peptides in C. albicans. Cells were exposed for 50 min to 2.5 μM of 5-FAM-peptides, counterstained with DAPI (blue) and visualized by confocal microscopy. Top and bottom panel show same images at lower and higher magnification, respectively. Short arrow: fragmented nuclear DNA; long arrow: tubular-shaped DNA, in contrast to single and round nucleus observed in untreated cells. Images are representatives of three independent experiments, in duplicate. Scale bars are 10 μm.en_US
dc.description.abstractPlease read abstract in the article.en_US
dc.description.departmentAnatomyen_US
dc.description.departmentBiochemistryen_US
dc.description.departmentGeneticsen_US
dc.description.departmentMicrobiology and Plant Pathologyen_US
dc.description.embargo2022-12-05
dc.description.librarianhj2022en_US
dc.description.sponsorshipUniversity of Pretoria; National Research Foundation; South African Medical Research Council.en_US
dc.description.urihttp://wileyonlinelibrary.com/journal/pscen_US
dc.identifier.citationMbuayama, K.R., Taute, H., Stromstedt, A.A., Bester, M.J. & Gaspar, A.R.M. Antifungal activity and mode of action of synthetic peptides derived from the tick OsDef2 defensin. Journal of Peptide Science 2022;28(5):e3383.doi:10.1002/psc.3383.en_US
dc.identifier.issn1075-2617 (print)
dc.identifier.issn1099-1387 (online)
dc.identifier.other10.1002/psc.3383
dc.identifier.urihttps://repository.up.ac.za/handle/2263/86414
dc.language.isoenen_US
dc.publisherWileyen_US
dc.rights© 2021 European Peptide Society and John Wiley & Sons, Ltd. This is the pre-peer reviewed version of the following article : Antifungal activity and mode of action of synthetic peptides derived from the tick OsDef2 defensin. Journal of Peptide Science 2022;28(5):e3383.doi:10.1002/psc.3383, which has been published in final form at : http://wileyonlinelibrary.com/journal/psc.en_US
dc.subjectAntimicrobial peptidesen_US
dc.subjectDefensinsen_US
dc.subjectMembrane permeabilizationen_US
dc.subjectReactive oxygen species (ROS)en_US
dc.subjectTicksen_US
dc.titleAntifungal activity and mode of action of synthetic peptides derived from the tick OsDef2 defensinen_US
dc.typePostprint Articleen_US

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