Abstract:
In this study, Brucella melitensis infection in sable antelope (Hippotragus niger) was investigated
on two wildlife ranches in South Africa over a 12-year period in order to determine the origin of the
outbreaks and the role of livestock in maintaining the disease. Retrospective data were obtained from
farm records and interviews as well as samples tested from di erent disease scenarios and clinical
settings. On one ranch, 10 of 74 sable tested seropositive for B. melitensis in 2004 but were certified clear
of infection after no further brucellosis cases were detected following repeated serological tests and
culling over a five-year period. Recrudescence occurred in 2013 (7 of 187 brucellosis positives) and in
2014 (one positive), with persistent, latent infection being the most reasonable explanation. In a second
case study, linked to the first one through a common vendor, 15 of 80 sable tested positive in 2016,
some five years after the acquisition of the animals from a putative source. Brucella melitensis biovar
1 and/or 3 were isolated from each outbreak on both ranches. Both outbreaks resulted in substantial
losses for the owners, arising from testing and culling and significant resource expenditure by the
state. The study identified the diagnostic challenges for identifying and resolving disease outbreaks in
wildlife, the persistence of B. melitensis in sable, the risks associated with animal movements, and the
need for a wildlife-sensitive disease control scheme. Although the actual source of infection could not
be identified, the investigation points away from local livestock as a source of ongoing infection while
the persistent infection is consistent with the disease circulating within small, ranched populations
and being spread through the keeping and trading of high-value animals. The implications of the
study findings to disease control in wildlife are discussed.
