dc.contributor.author |
Nothling, Johan Oliviette
|
|
dc.contributor.author |
De Cramer, Kurt Guido Mireille
|
|
dc.date.accessioned |
2021-02-04T06:10:01Z |
|
dc.date.available |
2021-02-04T06:10:01Z |
|
dc.date.issued |
2019-10-22 |
|
dc.description.abstract |
The Coat-A-Count® radioimmunoassay has been long and widely used to determine the
concentration of progesterone in serum or plasma of bitches (progRIA), but was discontinued
in 2014. The Immulite® 1000 LKPG1 chemiluminescence immunoassay has gained prominence
since 2003 to determine the concentration of progesterone in serum of bitches, but the assay
changed in 2012 (Immulite® 1000 LKPW1). This study assessed the feasibility of using
Immulite® 1000 LKPW1 (progImm) to estimate the time of clinically relevant events during
oestrus and compared progRIA and progImm 2 and 3 days after the first or only day of the
luteinizing hormone surge (LH1). ProgImm first exceeded 5.1 nmol/L on the same day that
progRIA first exceeded 6 nmol/L, a proxy for the occurrence of the LH surge, or the day before
in 28 of 31 (90%) of oestrous periods. ProgImm first exceeded 13.6 nmol/L on the same day
that progRIA first exceeded 16 nmol/L (a proxy for the day of ovulation) or the day before in
34 of 35 (97%) oestrous periods. ProgImm first exceeded 5.4 nmol/L on LH1 or the day before
in 24 of 25 (95%) of oestrous periods. The median of progImm 2 days after LH1 was 1.2 nmol/L
lower than the 10.7 nmol/L of progRIA (p = 0.001). The mean of progImm 3 days after LH1 was
2.2 nmol/L lower than the 19.0 nmol/L of progRIA (p < 0.001). In conclusion, the days on
which progImm first exceeded 5.1 nmol/L, 13.6 nmol/L and 5.4 nmol/L effectively estimate
the days on which progRIA reached 6 nmol/L or 16 nmol/L or LH1. |
en_ZA |
dc.description.department |
Production Animal Studies |
en_ZA |
dc.description.sponsorship |
The National Research Foundation |
en_ZA |
dc.description.uri |
http://www.jsava.co.za |
en_ZA |
dc.identifier.citation |
Nöthling, J.O. & De Cramer,
K.G.M., 2019, ‘Comparison of
progesterone assay by
chemiluminescence or
radioimmunoassay for clinical
decision-making in canine
reproduction’, Journal of the
South African Veterinary
Association 90(0), a1730.
https://DOI.org/10.4102/jsava.v90i0.1730. |
en_ZA |
dc.identifier.issn |
1019-9128 (print) |
|
dc.identifier.issn |
2224-9435 (online) |
|
dc.identifier.other |
10.4102/jsava.v90i0.1730 |
|
dc.identifier.uri |
http://hdl.handle.net/2263/78246 |
|
dc.language.iso |
en |
en_ZA |
dc.publisher |
AOSIS OpenJournals |
en_ZA |
dc.rights |
© 2019. The Authors.
Licensee: AOSIS. This work
is licensed under the
Creative Commons
Attribution License. |
en_ZA |
dc.subject |
Progesterone |
en_ZA |
dc.subject |
Bitch |
en_ZA |
dc.subject |
Radioimmunoassay |
en_ZA |
dc.subject |
Coat-a-count |
en_ZA |
dc.subject |
Immulite |
en_ZA |
dc.subject |
Luteinizing hormone |
en_ZA |
dc.title |
Comparison of progesterone assay by chemiluminescence or radioimmunoassay for clinical decision-making in canine reproduction |
en_ZA |
dc.type |
Article |
en_ZA |