Abstract:
The Coat-A-Count® radioimmunoassay has been long and widely used to determine the
concentration of progesterone in serum or plasma of bitches (progRIA), but was discontinued
in 2014. The Immulite® 1000 LKPG1 chemiluminescence immunoassay has gained prominence
since 2003 to determine the concentration of progesterone in serum of bitches, but the assay
changed in 2012 (Immulite® 1000 LKPW1). This study assessed the feasibility of using
Immulite® 1000 LKPW1 (progImm) to estimate the time of clinically relevant events during
oestrus and compared progRIA and progImm 2 and 3 days after the first or only day of the
luteinizing hormone surge (LH1). ProgImm first exceeded 5.1 nmol/L on the same day that
progRIA first exceeded 6 nmol/L, a proxy for the occurrence of the LH surge, or the day before
in 28 of 31 (90%) of oestrous periods. ProgImm first exceeded 13.6 nmol/L on the same day
that progRIA first exceeded 16 nmol/L (a proxy for the day of ovulation) or the day before in
34 of 35 (97%) oestrous periods. ProgImm first exceeded 5.4 nmol/L on LH1 or the day before
in 24 of 25 (95%) of oestrous periods. The median of progImm 2 days after LH1 was 1.2 nmol/L
lower than the 10.7 nmol/L of progRIA (p = 0.001). The mean of progImm 3 days after LH1 was
2.2 nmol/L lower than the 19.0 nmol/L of progRIA (p < 0.001). In conclusion, the days on
which progImm first exceeded 5.1 nmol/L, 13.6 nmol/L and 5.4 nmol/L effectively estimate
the days on which progRIA reached 6 nmol/L or 16 nmol/L or LH1.
