Detection of the Janus kinase 2 V617F mutation using a locked nucleic-acid, real-time polymerase chain reaction assay
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| dc.contributor.author | Senamela, Tshiphiri
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| dc.contributor.author | Kock, Marleen M.
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| dc.contributor.author | Becker, Piet J.
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| dc.contributor.author | Potgieter, Joachim
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| dc.date.accessioned | 2019-04-17T09:00:10Z | |
| dc.date.available | 2019-04-17T09:00:10Z | |
| dc.date.issued | 2018-01-31 | |
| dc.description.abstract | The purpose of this study was to develop a real time polymerase chain reaction (PCR) assay for the detection of the JAK2 V617F mutation that could be used in diagnostic laboratories. Sanger sequencing and a newly developed locked nucleic-acid, real-time PCR assay were used to detect the JAK2 V617F mutation. There was 100% agreement between the sequencing and PCR analysis. Both assays were able to detect the mutation in all 24 of the 60 test specimens harbouring the mutation. | en_ZA |
| dc.description.department | Haematology | en_ZA |
| dc.description.librarian | am2019 | en_ZA |
| dc.description.sponsorship | The National Health Laboratory Service research trust | en_ZA |
| dc.description.uri | http://www.ajlmonline.org | en_ZA |
| dc.identifier.citation | Senamela T, Kock M, Becker P, et al. Detection of the Janus kinase 2 V617F mutation using a locked nucleic-acid, real-time polymerase chain reaction assay. African Journal of Laboratory Medicine 2018;7(1), a658. https://DOI.org/10.4102/ajlm.v7i1.658. | en_ZA |
| dc.identifier.issn | 2225-2002 (print) | |
| dc.identifier.issn | 2225-2010 (online) | |
| dc.identifier.other | 10.4102/ajlm.v7i1.658 | |
| dc.identifier.uri | http://hdl.handle.net/2263/68993 | |
| dc.language.iso | en | en_ZA |
| dc.publisher | AOSIS Open Journals | en_ZA |
| dc.rights | © 2018. The Authors. Licensee: AOSIS. This work is licensed under the Creative Commons Attribution License. | en_ZA |
| dc.subject | Diagnostic laboratories | en_ZA |
| dc.subject | Sequencing | en_ZA |
| dc.subject | Polymerase chain reaction (PCR) | en_ZA |
| dc.subject | Detection | en_ZA |
| dc.subject | Janus kinase 2 V617F mutation | en_ZA |
| dc.title | Detection of the Janus kinase 2 V617F mutation using a locked nucleic-acid, real-time polymerase chain reaction assay | en_ZA |
| dc.type | Article | en_ZA |
