The purpose of this study was to develop a real time polymerase chain reaction (PCR) assay
for the detection of the JAK2 V617F mutation that could be used in diagnostic laboratories.
Sanger sequencing and a newly developed locked nucleic-acid, real-time PCR assay were used
to detect the JAK2 V617F mutation. There was 100% agreement between the sequencing and
PCR analysis. Both assays were able to detect the mutation in all 24 of the 60 test specimens
harbouring the mutation.