S1 Fig. Domains and classes for glycoside hydrolase 18 and 19 (chitinases) from the Eucalyptus grandis sequences. S = signal sequence, H = hinge region (proline/threonine-rich in Class IV and glycine-rich in Class I), CBD = chitin binding domain, black box indicates C-terminal vacuolar extension, aa = amino acid residue with approximate sizes. Diagrammatic concept from Collinge, Kragh et al. (1993).
S2 Fig. Quantitative reverse-transcriptase polymerase chain reaction relative expression change (A) of putative Class 1A chitinase (Eucgr. I01495) in Eucalyptus grandis in response to Chrysoporthe austroafricana inoculation (three days post-inoculation). Expression is relative to reference gene Elongation factor S-II (B). R = resistant, S = susceptible.
S3 Fig. Predicted tertiary structure for (A) Eucalyptus grandis Class IA chitinase peptide with cleaved signal and vacuolar sequences had a C-score of 1.72 (with range being between 5 and 2) and estimated TM-score of 0.96 (structural similarity score between 0 and 1) (Roy et al. 2010). (B) The putative mature protein matched tertiary structure for Oryza sativa L. japonica Class IA chitinase crystal structure (Kezuka et al. 2010) with TM score of 0.95 (http://www.rcsb.org/pdb/explore/jmol.do?structureId=2DKV&bionumber=1&opt=3&jmolMod e=HTML5). Blue = GH19 and Chitin binding domains (linked by hinge region), white = hinge region (glycine-rich in E.grandis Class I but usually proline/threonine rich), red = catalytic regions as identified by 0.6nm of bound substrate, magenta = essential residues for catalytic activity determined with mutagenesis (Bishop et al. 2000).
S1 Table. Full list of Eucalyptus grandis putative chitinase genes sorted by position on the genome. Information per gene includes the chromosomal position, class, physical cluster and localization. Log2 of normalized FPKM reads expression data, log2 fold change values and ANOVA results (p values). S_F_C = susceptible, fungal treatment, control. S_F_I = susceptible, fungal treatment, inoculated. R_F_C = resistant, fungal treatment, control. R_F_I = resistant, fungal treatment, inoculated. S_I_C = susceptible, insect treatment, control. S_I_I = susceptible, insect treatment, infested. R_I_C = resistant, insect treatment, control. R_I_I = resistant, insect treatment, infested.