dc.contributor.author |
Yunker, C.E.
|
|
dc.contributor.author |
Kocan, K.M.
|
|
dc.contributor.author |
Norval, R.A.I.
|
|
dc.contributor.author |
Burridge, M.J.
|
|
dc.contributor.editor |
Bigalke, R.D. |
|
dc.date.accessioned |
2017-11-13T09:18:58Z |
|
dc.date.available |
2017-11-13T09:18:58Z |
|
dc.date.created |
2017 |
|
dc.date.issued |
1987 |
|
dc.description |
The articles have been scanned in colour with a HP Scanjet 5590; 600dpi.
Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format. |
en_ZA |
dc.description.abstract |
Mallory's phloxine-methylene blue stain was used to differentiate colonies of Cowdria ruminantium in
midgut epithelial cells of nymphal Amblyomma hebraeum that had been infected as larvae. Gut tissues were
collected from nymphs that had fed on a susceptible sheep and were fixed in formol-saline on the day of
repletion. Paraffin sections, 3-4 μm thick, were then stained and this rendered colonies and cell nuclei densely
blue against a uniformly pink background of tick tissues. Colonies were easily distinguished from nuclei by their
specific morphology. This method of parasite visualization may be adapted to field-collected ticks for rapid
detection of C. ruminantium or to assays of susceptibility of tick populations to various strains of the organism. |
en_ZA |
dc.identifier.citation |
Yunker, CE, Kocan, KM, Norval, RAI & Burridge, MJ 1987, 'Distinctive staining of colonies of Cowdria ruminantium in midguts of Amblyomma hebraeum', Onderstepoort Journal of Veterinary Research, vol. 54, no. 3, pp. 183-185. |
en_ZA |
dc.identifier.issn |
0330-2465 |
|
dc.identifier.uri |
http://hdl.handle.net/2263/63116 |
|
dc.language.iso |
en |
en_ZA |
dc.publisher |
Published by the Government Printer, Pretoria. |
en_ZA |
dc.rights |
©ARC - Onderstepoort and Faculty of Veterinary Science, University of Pretoria (original). ©University of Pretoria. Dept. of Library Services (digital). |
en_ZA |
dc.subject |
Veterinary medicine |
en_ZA |
dc.subject.lcsh |
Veterinary medicine -- South Africa |
|
dc.title |
Distinctive staining of colonies of Cowdria ruminantium in midguts of Amblyomma hebraeum |
en_ZA |
dc.title.alternative |
Heartwater : past, present and future : proceedings of a workshop held at Berg en Dal, Kruger National Park, on 8-16 September 1986 |
en_ZA |
dc.type |
Article |
en_ZA |