Molecular identification of different Theileria and Babesia species infecting sheep in Sudan

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dc.contributor.author El Imam, Ahmed H.
dc.contributor.author Hassan, Shawgi M.
dc.contributor.author Gameel, Ahmed A.
dc.contributor.author El Hussein, Abdelrahim M.
dc.contributor.author Taha, Khalid M.
dc.contributor.author Oosthuizen, Marinda C.
dc.date.accessioned 2016-09-12T14:07:49Z
dc.date.available 2016-09-12T14:07:49Z
dc.date.issued 2016
dc.description.abstract The epidemiological aspects of sheep piroplasmosis in Sudan are poorly studied, and further investigations using sensitive and precise techniques are required. In this study, the Reverse Line Blot (RLB) hybridization assay was used to detect and simultaneously differentiate between Theileria and Babesia species. DNA was extracted from blood collected on filter paper (n=219) from apparently healthy sheep from six different geographical localities in Sudan. Results indicated that Theileria ovis (88.6%), T. separata (20.1%), T. lestoquardi (16.4%) and T. annulata (16.4%) DNA could be detected in the blood samples. Single and mixed Theileria infections were detected in 74 (33.8%) and 124 (56.6%) respectively and T. ovis being the most prevalent species in the country. T. ovis and T. separata were reported for the first time in sheep in Sudan. en_ZA
dc.description.department Veterinary Tropical Diseases en_ZA
dc.description.librarian am2016 en_ZA
dc.description.sponsorship University of Elimam Elmahadi, Ministry of High Education and Scientific Research, Sudan en_ZA
dc.description.uri http://www.bioone.org/loi/copa en_ZA
dc.identifier.citation El Imam, AH, Hassan, SM, Gameel, AA, El Hussein, AM, Taha, KM & Oosthuizen, MC 2016, 'Molecular identification of different Theileria and Babesia species infecting sheep in Sudan', Annals of Parasitology, pp. 62, no. 1, pp. 47-54. en_ZA
dc.identifier.issn 2299-0631 (print)
dc.identifier.issn 2300-6706 (online)
dc.identifier.other 10.17420/ap6201.31
dc.identifier.uri http://hdl.handle.net/2263/56722
dc.language.iso en en_ZA
dc.publisher Polish Parasitological Society en_ZA
dc.rights © 2016 Polish Parasitological Society en_ZA
dc.subject Sudan en_ZA
dc.subject Theileria annulata en_ZA
dc.subject Theileria lestoquardi en_ZA
dc.subject Theileria ovis en_ZA
dc.subject Theileria separata en_ZA
dc.subject Reverse line blot (RLB) en_ZA
dc.title Molecular identification of different Theileria and Babesia species infecting sheep in Sudan en_ZA
dc.type Article en_ZA


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