S1 Text. Explanation of the development of a fluorescent cell biological evaluation technique
to detect intra-erythrocytic B. divergens parasites.
S1 Fig. Flow cytometric analysis of intra-erythrocytic B. divergens parasites. Uninfected
erythrocytes were analysed in parallel to B. divergens infected erythrocytes, either fixed
(0.025% glutaraldehyde for 45 min) or unfixed. Cells were subsequently stained with either
1:100 and 1:1000 SYBR Green I (30 min, dark, room temperature). (A) Dotblot analysis and
(B) histograms of 1) uninfected, unfixed, stained erythrocytes; 2–5) B. divergens infected erythrocytes
either unfixed (2 & 3) or fixed (4 & 5). In panels 2 and 4 cells were stained with 1:100
SYBR Green I and in panels 3 and 5 with 1:1000 SYBR Green I. Erythrocytes infected with P.
falciparum parasites were comparatively analysed in panel 6 (glutaraldehyde fixed and stained
with 1:1000 SYBR Green I. (C) Effect of SYBR Green I concentrations on parasitemia determined
from both unfixed and fixed B. divergens infected erythrocytes. Results are the mean of
three independent experiments each performed in triplicate (± S.E.). Significance is indicated
at P<0.001 ( ) (unpaired Student-t test). (D) Linear correlation analysis (R2 value of 0.98) of B.
divergens parasitemia detection between light microscopy and flow cytometry. Data are the
mean of three independent experiments each performed in triplicate (± S.E.). Confidence levels
(95%) indicated by dashed lines.
S1 Table. Differentially affected transcripts identified in the initiate culture before culture
adaptation.