dc.contributor.author |
Heartwater : Past, Present and Future. Workshop (1986 : Kruger National Park, South Africa)
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dc.contributor.author |
Viljoen, Gerrit Johannes
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dc.contributor.author |
Vermeulen, N.M.J.
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dc.contributor.editor |
Bigalke, R.D. |
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dc.contributor.upauthor |
Neitz, Albert Walter Herman
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dc.date.accessioned |
2014-11-19T10:18:25Z |
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dc.date.available |
2014-11-19T10:18:25Z |
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dc.date.created |
2014 |
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dc.date.issued |
1987 |
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dc.description |
The articles have been scanned in colour with a HP Scanjet 5590; 600dpi.
Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format. |
en_US |
dc.description.abstract |
Numerous parameters affect the enzyme-linked immunosorbent assay activity and the assay conditions must therefore be carefully controlled to obtain reproducible results. These parameters include temperature, pH, ionic strength, buffer composition, cofactors, substrate depletion, product inhibition, increasing reversal of reaction as product concentration increases, adsorption of enzyme to solid supports, denaturation and non-enzymatic background rate. An ELISA was used to detect Cowdria ruminantium antibodies during the course of heartwater disease. lgM antibodies reached a maximum on the 4th day after infection and disappeard on the 7th day. IgG antibodies first appeared on the 8th day and continued to increase during the remainder of the observation period of 28 days. The presence of C. ruminantium in the blood fractions of diseased animals was demonstrated by an enzyme-linked immunosorbent assay. The earliest detection of C. ruminantium antigen was in plasma and serum on the 4th day after inoculation. Of all the blood fractions investigated, the red blood cell fraction showed the highest concentration and this reached a maximum on the 12th day after infection. |
en_US |
dc.description.librarian |
lmchunu2014 |
en_US |
dc.description.librarian |
mn2014 |
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dc.identifier.citation |
Viljoen, GJ, Vermeulen, NMJ & Neitz, AWH 1987, 'Theoretical aspects of the enzyme-linked immunosorbent assay technique and its use in the detection of Cowdria ruminantium antigen and antibody in reacting animals’, Onderstepoort Journal of Veterinary Research, vol. 54, no. 3, pp. 305-312. |
en_US |
dc.identifier.issn |
0330-2465 |
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dc.identifier.uri |
http://hdl.handle.net/2263/42664 |
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dc.language.iso |
en |
en_US |
dc.publisher |
Published by the Government Printer, Pretoria. |
en_US |
dc.rights |
©ARC - Onderstepoort and Faculty of Veterinary Science, University of Pretoria (original). ©University of Pretoria. Dept. of Library Services (digital). |
en_US |
dc.subject |
Veterinary medicine |
en_US |
dc.subject.lcsh |
Veterinary medicine -- South Africa |
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dc.subject.lcsh |
Heartwater -- South Africa |
en |
dc.title |
Theoretical aspects of the enzyme-linked immunosorbent assay technique and its use in the detection of Cowdria ruminantium antigen and antibody in reacting animals |
en_US |
dc.title.alternative |
Heartwater : past, present and future : proceedings of a workshop held at Berg en Dal, Kruger National Park, on 8-16 September 1986 |
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dc.type |
Article |
en_US |