Protection against heartwater by DNA immunisation with four Ehrlichia ruminantium open reading frames

Pretorius, Alri; Collins, Nicola E.; Steyn, H.C.; Van Strijp, M.F.; Van Kleef, Mirinda; Allsopp, Basil A.

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dc.contributor.author	Pretorius, Alri
dc.contributor.author	Collins, Nicola E.
dc.contributor.author	Steyn, H.C.
dc.contributor.author	Van Strijp, M.F.
dc.contributor.author	Van Kleef, Mirinda
dc.contributor.author	Allsopp, Basil A.
dc.date.accessioned	2007-07-24T07:23:27Z
dc.date.available	2007-07-24T07:23:27Z
dc.date.issued	2007-03-08
dc.description.abstract	We have reported previously that a recombinant DNA vaccine consisting of four Ehrlichia ruminantium (Welgevonden) open reading frames (ORFs) known as the 1H12 cocktail provided protection against a virulent E. ruminantium (Welgevonden) needle challenge in sheep. In this study, we have investigated the vaccine effectiveness of two other cocktails of E. ruminantium (Welgevonden) ORFs, as well as single ORFs from the 1H12 cocktail, to protect sheep against a virulent needle challenge with the homologous strain. Each individual 1H12 ORF provided protection, but all the animals vaccinated with the other cocktails succumbed to the challenge.	en
dc.description.sponsorship	The authors thank Stephen Johnston (University of Texas Southwestern Medical Center) for supplying the pCMViUBs vector and Dr. Erich Zweygarth and Antoinette Josemans (ARC-Onderstepoort Veterinary Institute, SA) for providing E. ruminantium tissue culture material for DNA extraction. This work was supported by the South African Department of Science and Technology LEAD biotechnology grant and the EU INCO-ICA4-CT-2000-30026 grant.	en
dc.format.extent	341437 bytes
dc.format.mimetype	application/pdf
dc.identifier.citation	Pretorius, A, Collins, NE, Steyn, HC, Van Strijp, F, Van Kleef, M & Allsopp, BA 2007, ‘Protection against heartwater by DNA immunisation with four Ehrlichia ruminantium open reading frames’, Vaccine, vol. 25, no. 12, pp. 2316-2324.[http://www.sciencedirect.com/science/journal/0264410X]	en
dc.identifier.issn	0264-410X
dc.identifier.other	10.1016/j.vaccine.2006.11.061
dc.identifier.other	7103250386
dc.identifier.other	O-6342-2014
dc.identifier.uri	http://hdl.handle.net/2263/3114
dc.language.iso	en	en
dc.publisher	Elsevier	en
dc.rights	Elsevier	en
dc.subject	Ehrlichia ruminantium	en
dc.subject	DNA vaccination	en
dc.subject	ABC transporters	en
dc.subject.lcsh	Heartwater -- Vaccination	en
dc.subject.lcsh	Sheep -- Diseases	en
dc.title	Protection against heartwater by DNA immunisation with four Ehrlichia ruminantium open reading frames	en
dc.type	Postprint Article	en