Molecular and serological detection of A. centrale- and A. marginale-infected cattle grazing within an endemic area

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dc.contributor.author Molad, T.
dc.contributor.author Mazuz, M.L.
dc.contributor.author Fleiderovitz, L.
dc.contributor.author Fish, Lyn
dc.contributor.author Savitsky, I.
dc.contributor.author Krigel, Y.
dc.contributor.author Leibovitz, B.
dc.contributor.author Molloy, J.
dc.contributor.author Jongejan, Frans
dc.contributor.author Shkap, Varda
dc.date.accessioned 2007-05-11T07:20:02Z
dc.date.available 2007-05-11T07:20:02Z
dc.date.issued 2006
dc.description.abstract A reverse line blot hybridization (RLB) one-stage nested PCR (nPCR) for Anaplasma centrale and a nested PCR for Anaplasma marginale were used to detect infected cattle grazing within an endemic region in Israel. A novel set of PCR primers and oligonucleotide probes based on a 16S ribosomal RNA gene was designed for RLB detection of both Anaplasma species, and the performance of the molecular assays compared. The immunofluorescent antibody test (IFA) was used to detect antibodies to both Anaplasma species, whereas, a highly sensitive and specific competitive enzyme-linked immunosorbent assay (cELISA) was used to detect antibodies in A. centrale-vaccinated cattle. The RLB and the nested PCR procedures showed bacteremia with sensitivity of 50 infected erythrocytes per milliliter. Up to 93% of the A. centrale vaccinates carried specific antibodies that were detected by cELISA, and up to 71% of the vaccinated cattle were found to be naturally infected with A. marginale according to the PCR and the RLB assays. Nevertheless, no severe outbreaks of A. marginale infection occurred among vaccinated herds in this endemic region. It appears that both, molecular tools and serology are useful for evaluation of the vaccine efficacy. In the light of wide natural field infection with A. marginale, strong recommendations to continue the A. centrale vaccination program regime will continue until a new generation of non-blood-based vaccine will be developed. en
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dc.identifier.citation Molad, T, Mazuz, ML, Fleiderovitz, L, Fish, L, Savitsky, I, Krigel, Y, Leibovitz, B, Molloy, J, Jongejan, F & Shkap, V 2006. ‘Molecular and serological detection of A. centrale- and A. marginale-infected cattle grazing within an endemic area’, Veterinary Microbiology, vol. 113, issues 1-2, pp.55-62 [http://www.sciencedirect.com/science/journal/03781135 ] en
dc.identifier.issn 0378-1135
dc.identifier.other 10.1016/j.vetmic.2005.10.026
dc.identifier.uri http://hdl.handle.net/2263/2360
dc.language.iso en en
dc.publisher Elsevier en
dc.rights Elsevier en
dc.subject Serology en
dc.subject Vaccination en
dc.subject Reverse blot line hybridization en
dc.subject.lcsh Anaplasma marginale en
dc.subject.lcsh Anaplasma en
dc.subject.lcsh Cattle -- Vaccination en
dc.subject.lcsh Cattle -- Infections en
dc.title Molecular and serological detection of A. centrale- and A. marginale-infected cattle grazing within an endemic area en
dc.type Postprint Article en


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