Browsing 2002 Onderstepoort Journal of Veterinary Research, Volume 69, 2002 by Title

Browsing 2002 Onderstepoort Journal of Veterinary Research, Volume 69, 2002 by Title

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  • Description of Pelonia africana n. g., n. sp. (Pentastomida: Sebekidae) from the lungs of Pelomedusa subrufa and Pelusios sinuatus (Chelonia) in South Africa 
    Boomker, Jacob Diederik Frederik; Junker, Kerstin; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    The terrapins Pelomedusa subrufa and Pelusios sinuatus taken from a water reservoir in the Northern Province, South Africa, were examined for pentastome infections. Two immature specimens, a patent female and a mature male, ...
  • Development of a diagnostic one-tube RT-PCR for the detection of Rift Valley fever virus 
    Nel, Louis Hendrik; Espach, Anel; Romito, M.; Viljoen, G.J.; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    Diagnosis of Rift Valley fever (RVF) is based on serology and virus isolation. The disadvantages of the former include poor sensitivity, high cost, risks associated with using infectious virus as antigen, the lengthy ...
  • Development of solid phase antigen for indirect ELISA for the detection of specific antibody responses to infection with Newcastle disease virus 
    Sahle, M.; Burgess, G.W.; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    A simple and inexpensive method of antigen preparation by ultrafiltration was investigated using the V4 strain of Newcastle disease virus. The antigen designated XM300 was used in an indirect enzyme-linked immunosorbent ...
  • The effect of three bufadienolide cardiac glycosides on contraction of isolated rat jejunum 
    Botha, C.J. (Christoffel Jacobus); Gehring, Ronette; Van Rooyen, J.M.; Venter, D.; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    Three cardiac glycosides were screened for pharmacological effects on isolated rat jejunum. The contraction of rat jejunum with epoxyscillirosidin, a non-cumulative bufadienolide, and cotyledoside and tyledoside D, both ...
  • Effects of the scab mite Psoroptes ovis on the haematology and live mass of Merino and Dorper sheep 
    Horak, Ivan Gerard; Meintjes, T.; Fourie, L.J.; Boomker, Jacob Diederik Frederik (Pretoria : Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    Five Merino and five Dorper sheep were artificially infested with the sheep scab mite Psoroptes ovis and the effect of infestation on their haematology, serum protein levels and live mass recorded for a period of 14 weeks. ...
  • Freezing of sheep faeces invalidates Haemonchus contortus faecal egg counts by the McMaster technique 
    Van Wyk, Jan Aucamp; Van Wyk, Laetitia; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    Faecal pellets from a sheep that was artificially infected with a monoculture of Haemonchus contortus were collected over a 2-h period in the morning. In the laboratory the faeces were thoroughly mixed by hand and 48 by 1 ...
  • The gamma-interferon test: its usefulness in a bovine tuberculosis survey in African buffaloes ( Syncerus caffer) in the Kruger National Park 
    Michel, Anita Luise; Grobler, D.G.; De Klerk, L.M.; Bengis, Roy G.; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    A survey to determine the bovine tuberculosis status of buffalo herds north of the Olifants River in the Kruger National Park was conducted, using a new diagnostic approach. Diagnosis of Mycobacterium bovis infection was ...
  • Hepatic and renal concentrations of copper and other trace elements in hippopotami (Hippopotamus amphibius L.) living in and adjacent to the Kafue and Luangwa Rivers in Zambia 
    Mwase, M.; Almli, B.; Sivertsen, T.; Musonda, M.M.; Flaoyen, A.; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    Hepatic and renal concentrations of the elements arsenic, cadmium, cobalt, copper, lead, manganese, mercury, molybdenum, selenium and zinc were studied in samples collected from hippopotami from the Kafue River in the Kafue ...
  • Hosts, seasonal occurrence and life cycle of Rhipicentor nuttalli (Acari: Ixodidae) 
    Horak, Ivan Gerard; Fourie, L.J.; Kok, D.J.; Van Zyl, W.; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    There are only two species worldwide within the genus Rhipicentor, namely Rhipicentor bicornis and Rhipicentor nuttalli, and both occur only in Africa. Rhipicentor nuttalli has a widespread distribution in South Africa and ...
  • In vitro isolation of equine piroplasms derived from Cape Mountain zebra (Equus zebra zebra) in South Africa 
    Zweygarth, Erich; Meyer, P.; Lopez-Rebollar, Laura M.; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    Twenty blood samples of zebras ( Equus zebra zebra) from the Karoo National Park and the Bontebok National Park in South Africa, all seropositive for Theileria equi, were subjected to in vitro culture to identify carrier ...
  • Isolation of serovar C-3 Haemophilus paragallinarum from Zimbabwe: a further indication of the need for the production of vaccines against infectious coryza containing local isolates of H. paragallinarum 
    Bragg, R.R. (Robert Richard); Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    Various isolates of Haemophilus paragallinarum, collected from a severe outbreak of infectious coryza in poultry from Zimbabwe, were serotyped and were found to belong to serovar C-3. Previously, isolates were serotyped ...
  • The Kumm isolate of Ehrlichia ruminantium: in vitro isolation, propagation and characterization 
    Zweygarth, Erich; Van Heerden, Henriette; Allsopp, Basil A.; Josemans, A.I.; Van Strijp, M.F.; Allsopp, M.T.E.P.; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    An effective culture system for Ehrlichia (Cowdria) ruminantium comb. nov. was first established in 1985 and many stocks were subsequently isolated and propagated in vitro. A notable exception, however, was the Kumm isolate ...
  • Multiplication of the V4 strain of Newcastle disease virus in Madin Derby bovine kidney cells 
    Sahle, M.; Burgess, W.G.; Kidanemariam, Awoke; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    This study describes a reproducible cell culture system that permits the growth and secondary multiplication of the V4 strain of Newcastle disease virus. Allantoic fluid, magnesium chloride and diethylaminoethyl dextran ...
  • Non-immune control of trypanosomosis: in vitro oxidative burst of PMA- and trypanosome-stimulated neutrophils of Boran and N'Dama cattle 
    Taiwo, V.O.; Adejinmi, J.O.; Oluwaniyi, J.O.; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    An in vitro assay that measures the generation of superoxide anions (O₂ˉ) was used to assess the level of oxidative burst of phorbol myristate acetate (PMA) - and trypanosome-stimulated neutrophils isolated from healthy ...
  • Occurrence of concurrent trypanosomosis, theileriosis, anaplasmosis and helminthosis in Friesian, Zebu and Sahiwal cattle in Uganda 
    Magona, Joseph W.; Mayende, J.S.P.; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    An epidemiological investigation was conducted on farms in Tororo and Soroti districts of Uganda from January to February 2000 to determine the cause of reported persistent mortality of cattle. Blood and faecal material ...
  • On-host ecology and off-host survival of the sheep scab mite Psoroptes ovis 
    Horak, Ivan Gerard; Meintjes, T.; Fourie, L.J.; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    These studies were conducted to investigate the possible role of certain aspects of the on-host ecology and off-host survival of the sheep scab mite, Psoroptes ovis, in the dissemination of infestation. All developmental ...
  • An overview of the eradication of Brucella melitensis from KwaZulu-Natal 
    Emslie, Forbes Richard; Nel, J.R.; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    Brucella melitensis is a Gram-negative bacterium whose primary hosts are goats and sheep. Like the other Brucella spp., with the exception of Brucella ovis, it is not particularly host specific as it is pathogenic for a ...
  • Parasitological prevalence of bovine trypanosomosis in Kindo Koisha district, Wollaita zone, south Ethiopia 
    Kidanemariam, Awoke; Hadgu, K.; Sahle, M.; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    A cross sectional survey to determine the distribution and prevalence of trypanosomosis was conducted in Kindo Koisha district, in the Wollaita zone in southern Ethiopia. A total of 1008 adult cattle was examined at eight ...
  • The prevalence and economic importance of bovine fasciolosis in Kenya - an analysis of abattoir data 
    Kithuka, J.M.; Maingi, N.; Njeruh, F.M.; Ombui, J.N.; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    A retrospective study covering a period of 10 years (1990-1999) was carried out using post mortem meat inspection records at the Veterinary Department Headquarters at Kabete to determine the prevalence and economic importance ...
  • Redescription of some Spauligodon spp. and Parapharyngodon spp., and of Skrjabinodon mabuyae (Sandground, 1936) Inglis, 1968 (Pharyngodonidae: Oxyuroidea) from insectivorous South African lizards 
    Boomker, Jacob Diederik Frederik; Hering-Hagenbeck, S.F.B.N.; Petter, A.J.; Boomker, Jacob Diederik Frederik (Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria., 2002)
    As part of a study on the helminth parasites of South African lizards several species of saurians were collected from localities in the North West Province, the Northern Province, Mpumalanga Province and Gauteng ...