dc.contributor.author |
Vorster, C.J.J. (Christiaan Jacob Johan)
|
|
dc.contributor.author |
Joubert, Annie M.
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|
dc.date.accessioned |
2011-05-12T07:33:31Z |
|
dc.date.available |
2011-05-12T07:33:31Z |
|
dc.date.issued |
2010-06 |
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dc.description.abstract |
In the search for new and improved anticancer therapies, researchers have identified several potentially useful compounds. One of these agents is 2-methoxyestradiol-bis-sulphamate (2ME-BM), a
sulphamoylated derivative of 2-methoxyestradiol. The objective of this study was to evaluate 2ME-BM’s in vitro efficacy as antiproliferative agent in the MCF-7 breast adenocarcinoma cell line. Light- and fluorescent microscopy showed decreased cell density, increased apoptotic characteristics and significant ultrastructural
aberrations indicative of autophagic cell death after 24 hours of exposure at a concentration of 0.4μM. In addition, mitotic indices revealed that 2ME-BM induces a G2M block. The latter was confirmed by flow cytometric analyses where increased sub-G1 and G2/M fractions, as well as an increase in cyclin B1 levels were observed. Further in vitro research into the mechanism of this potentially useful anticancer compound is thus warranted. |
en_US |
dc.description.sponsorship |
This research was supported by grants from the Medical Research Council of South Africa (AK076; AL343), the Cancer Association of South Africa (AK246), the National Research Foundation of South Africa (AL239) and the Struwig-Germeshuysen Cancer Research Trust of South Africa (AN074). |
en_US |
dc.identifier.citation |
Vorster, C & Joubert, A 2010, 'In vitro effects of 2-methoxyestradiol-bis-sulphamate on cell growth, morphology and cell cycle dynamics in the MCF-7 breast adenocarcinoma cell line', Biocell, vol. 34, no. 2, pp. 71-79. [http://www.scielo.org.ar/scielo.php/script_sci_serial/lng_en/pid_0327-9545/nrm_iso] |
en_US |
dc.identifier.issn |
0327-9545 (print) |
|
dc.identifier.issn |
1667-5746 (online) |
|
dc.identifier.uri |
http://hdl.handle.net/2263/16569 |
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dc.language.iso |
en |
en_US |
dc.publisher |
Centro Regional de Investigaciones Cientificas Y Tecnologicas / Universidad Nacional de Cuyo |
en_US |
dc.rights |
Centro Regional de Investigaciones Cientificas Y Tecnologicas / Universidad Nacional de Cuyo |
en_US |
dc.subject |
Cancer |
en_US |
dc.subject |
2-methoxyestradiol |
en_US |
dc.subject |
Sulphamoylated estradiol derivative |
en_US |
dc.subject |
Apoptosis |
en_US |
dc.subject |
Autophagy |
en_US |
dc.subject.lcsh |
Cancer -- Treatment -- Research |
en |
dc.title |
In vitro effects of 2-methoxyestradiol-bis-sulphamate on cell growth, morphology and cell cycle dynamics in the MCF-7 breast adenocarcinoma cell line |
en_US |
dc.type |
Article |
en_US |