Worldwide diversity of endophytic fungi and insects associated with dormant tree twigs
Franic, Iva; Prospero, Simone; Adamson, Kalev; Allan, Eric; Attorre, Fabio; Auger-Rozenberg, Marie Anne; Augustin, Sylvie; Avtzis, Dimitrios; Baert, Wim; Barta, Marek; Bauters, Kenneth; Bellahirech, Amani; Boron, Piotr; Braganca, Helena; Brestovansk, Tereza; Brurberg, May Bente; Burgess, Treena I.; Burokiene, Daiva; Cleary, Michelle; Corley, Juan; Coyle, David R .; Csoka, Gyorgy; Cerny, Karel; Davydenko, Kateryna; De Groot, Maarten; Diez, Julio Javier; Lehtijärvi, H. Tugba Dogmus; Drenkhan, Rein; Edwards, Jacqueline; Elsafy, Mohammed; Eotvos, Csaba Bela; Falko, Roman; Fan, Jianting; Feddern, Nina; Furjes-Miko, Agnes; Gossner, Martin M.; Grad, Bartłomiej; Hartmann, Martin; Havrdova, Ludmila; Horakova, Miriam Kadasi; Hrabetova, Marketa; Justesen, Mathias Just; Kacprzyk, Magdalena; Kenis, Marc; Kirichenko, Natalia; Kovac, Marta; Kramarets, Volodymyr; Lackovic, Nikola; Lantschner, Maria Victoria; Lazarevic, Jelena; Leskiv, Marianna; Li, Hongmei; Madsen, Corrie Lynne; Malumphy, Chris; Matosevic, Dinka; Matsiakh, Iryna; May, Tom W.; Meffert, Johan; Migliorini, Duccio; Nikolov, Christo; O ’Hanlon, Richard; Oskay, Funda; Paap, Trudy; Parpan, Taras; Piskur, Barbara; Ravn, Hans P eter; Richard, John; Ronse, Anne; Roques, Alain; Ruffner, Beat; Sivickis, Karolis; Soliani, Carolina; Talgo, Venche; Tomoshevich, Maria; Uimari, Anne; Ulyshen, Michael; Vettraino, Anna Maria; Villari, Caterina; Wang, Yongjun; Witzell, Johanna; Zlatkovic, Milica; Eschen, Rene
Date:
2022-03-01
Abstract:
International trade in plants and climate change are two of the main factors causing
damaging tree pests (i.e. fungi and insects) to spread into new areas. To mitigate these
risks, a large-scale assessment of tree-associated fungi and insects is needed. We present
records of endophytic fungi and insects in twigs of 17 angiosperm and gymnosperm genera,
from 51 locations in 32 countries worldwide. Endophytic fungi were characterized by highthroughput
sequencing of 352 samples from 145 tree species in 28 countries. Insects were
reared from 227 samples of 109 tree species in 18 countries and sorted into taxonomic
orders and feeding guilds. Herbivorous insects were grouped into morphospecies and were
identified using molecular and morphological approaches. This dataset reveals the diversity
of tree-associated taxa, as it contains 12,721 fungal Amplicon Sequence Variants and 208
herbivorous insect morphospecies, sampled across broad geographic and climatic gradients
and for many tree species. This dataset will facilitate applied and fundamental studies on the
distribution of fungal endophytes and insects in trees.
Description:
Code availability
R functions and databases used for generating the sample metadata are specified in the method section. A
customized pipeline used for quality filtering of the raw sequence data obtained from HTS, delineation into ASVs
and taxonomic classification of ASVs as described in Herzog et al.30 is available as a “ITS2.bash” file from the
Zenodo repository28.