The first space-related study of a kombucha multimicrobial cellulose-forming community : preparatory laboratory experiments

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dc.contributor.author Podolich O.
dc.contributor.author Zaets, I.
dc.contributor.author Kukharenko, O.
dc.contributor.author Orlovska, I.
dc.contributor.author Khirunenko, L.
dc.contributor.author Sosnin, M.
dc.contributor.author Haidak, A.
dc.contributor.author Shpylova, S.
dc.contributor.author Rohutskyy, I.
dc.contributor.author Kharina, A.
dc.contributor.author Skoryk, M.
dc.contributor.author Kremenskoy, M.
dc.contributor.author Klymchuk, D.
dc.contributor.author Demets, R.
dc.contributor.author De Vera, J.P.
dc.contributor.author Kozyrovska, N.
dc.contributor.upauthor Reva, Oleg N.
dc.date.accessioned 2017-07-17T06:08:09Z
dc.date.issued 2017-06
dc.description.abstract Biofilm-forming microbial communities are known as the most robust assemblages that can survive in harsh environments. Biofilm-associated microorganisms display greatly increased resistance to physical and chemical adverse conditions, and they are expected to be the first form of life on Earth or anywhere else. Biological molecules synthesized by biofilm -protected microbiomes may serve as markers of the nucleoprotein life. We offer a new experimental model, a kombucha multimicrobial culture (KMC), to assess a structural integrity of a widespread microbial polymer - cellulose - as a biosignature of bacteria-producers for the multipurpose international project "BIOlogical and Mars Experiment (BIOMEX)", which aims to study the vitality of pro- and eukaryotic organisms and the stability of organic biomolecules in contact with minerals to analyze the detectability of life markers in the context of a planetary background. In this study, we aimed to substantiate the detectability of mineralized cellulose with spectroscopy and to study the KMC macrocolony phenotype stability under adverse conditions (UV, excess of inorganics etc.). Cellulose matrix of the KMC macrocolony has been mineralized in the mineral-water interface under assistance of KMC-members. Effect of bioleached ions on the cellulose matrix has been visible, and the FT-IR spectrum proved changes in cellulose structure. However, the specific cellulose band vibration, confirming the presence of beta(1,4)-linkages between monomers, has not been quenched by secondary minerals formed on the surface of pellicle. The cellulose-based KMC macrocolony phenotype was in a dependence on extracellular matrix components (ionome, viriome, extracellular membrane vesicles), which provided its integrity and rigidness in a certain extent under impact of stressful factors. en_ZA
dc.description.department Business Management en_ZA
dc.description.embargo 2018-06-30
dc.description.librarian hj2017 en_ZA
dc.description.uri https://link.springer.com/journal/11084 en_ZA
dc.identifier.citation Podolich, O., Zaets, I., Kukharenko, O., Orlovska, I., Reva, Oleg N., Khirunenko, L., Sosnin, M., Haidak, A., Shpylova, S., Rohutskyy, I., Kharina, A., Skoryk, M., Kremenskoy, M., Klymchuk, D., Demets, R., de Vera, J. -P., Kozyrovska, N. The first space-related study of a kombucha multimicrobial cellulose-forming community : preparatory laboratory experiments. Origins of Life and Evolution of Biospheres (2017) 47: 169-185. doi:10.1007/s11084-016-9483-4. en_ZA
dc.identifier.issn 1573-0875 (online)
dc.identifier.issn 0169-6149 (print)
dc.identifier.other 10.1007/s11084-016-9483-4
dc.identifier.uri http://hdl.handle.net/2263/61358
dc.language.iso en en_ZA
dc.publisher Springer en_ZA
dc.rights © Springer Science+Business Media Dordrecht 2016. The original publication is available at : https://link.springer.com/journal/11084. en_ZA
dc.subject Biology and mars experiment (BIOMEX) en_ZA
dc.subject Kombucha multimicrobial community (KMC) en_ZA
dc.subject Biosignature en_ZA
dc.subject Biofilm en_ZA
dc.subject Bacterial cellulose en_ZA
dc.title The first space-related study of a kombucha multimicrobial cellulose-forming community : preparatory laboratory experiments en_ZA
dc.type Postprint Article en_ZA


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