dc.contributor.author |
Mohamed, Khaled M.
|
|
dc.contributor.author |
Cromarty, Allan Duncan
|
|
dc.contributor.author |
Steenkamp, Vanessa
|
|
dc.date.accessioned |
2015-09-10T08:29:57Z |
|
dc.date.available |
2015-09-10T08:29:57Z |
|
dc.date.issued |
2015-08 |
|
dc.description.abstract |
In some developing countries, p-phenylenediamine (PPD) is used in combination with Henna as hair
dye or skin decoration. A sensitive LC–MS/MS method was developed and validated for the simultaneous
determination of p-phenylenediamine (PPD) and its metabolites N-acetyl-p-phenylenediamine (MAPPD)
andN,N-diacetyl-p-phenylenediamine (DAPPD) inhumanblood. Acetanilide was used as an internal standard
(IS). The LC–MS/MS was operated under multiple reaction-monitoring mode using the electrospray
positive ionization technique. The transition ions m/z 109→92, m/z 151→92, m/z 193→92, and m/z
136→77 were selected for the quantification of PPD, MAPPD, DAPPD, and IS, respectively. The linear
range was 10–2000 ng/mL for all the compounds. The absolute recoveries were 51.94, 56.20 and 54.88%
for PPD, MAPPD and DAPPD, respectively. Intra- and inter-assay imprecision were lower than 14% (RSD),
and the bias of the assay was lower than 15% for all the compounds. The stability studies demonstrated
that critical degradation for PPD in blood samples and autosampler occurred after 6 h, while MAPPD and
DAPPD were stable in blood samples and the autosampler up to 48 h and 24 h, respectively. This newly
developed method allows for the detection of PPD and its metabolites in blood samples in the clinical
and forensic setting. |
en_ZA |
dc.description.embargo |
2016-08-31 |
en_ZA |
dc.description.librarian |
hb2015 |
en_ZA |
dc.description.sponsorship |
International Federation of Clinical Chemistry and Laboratory Medicine (IFCC). |
en_ZA |
dc.description.uri |
http://www.elsevier.com/locate/chromb |
en_ZA |
dc.identifier.citation |
Mohamed, KM, Cromarty, AD & Steenkamp, V 2015, 'Development and validation of an LC-MS/MS method for determination of p-phenylenediamine and its metabolites in blood samples', Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, vol. 997, pp. 1-6. |
en_ZA |
dc.identifier.issn |
1570-0232 (print) |
|
dc.identifier.issn |
1873-376X (online) |
|
dc.identifier.other |
10.1016/j.jchromb.2015.05.030 |
|
dc.identifier.uri |
http://hdl.handle.net/2263/49769 |
|
dc.language.iso |
en |
en_ZA |
dc.publisher |
Elsevier |
en_ZA |
dc.rights |
© 2015 Elsevier B.V. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, vol. 997, pp. 1-6, 2015. doi : 10.1016/j.jchromb.2015.05.030. |
en_ZA |
dc.subject |
Metabolites |
en_ZA |
dc.subject |
Blood |
en_ZA |
dc.subject |
LC–MS/MS |
en_ZA |
dc.subject |
Validation |
en_ZA |
dc.subject |
Stability |
en_ZA |
dc.subject |
p-phenylenediamine (PPD) |
en_ZA |
dc.title |
Development and validation of an LC-MS/MS method for determination of p-phenylenediamine and its metabolites in blood samples |
en_ZA |
dc.type |
Postprint Article |
en_ZA |