dc.contributor.author |
Williams, Aurelia Alvina
|
|
dc.contributor.author |
Steffens, Francois E.
|
|
dc.contributor.author |
Reinecke, Carolus J.
|
|
dc.contributor.author |
Meyer, Debra
|
|
dc.date.accessioned |
2013-06-04T11:45:18Z |
|
dc.date.available |
2013-06-04T11:45:18Z |
|
dc.date.issued |
2013-02 |
|
dc.description.abstract |
HIV infection causes the dysregulation of cytokine production. A cytokinomics approach
employing cytometric bead array (CBA) technology, flow cytometry and multivariate analysis
was applied to the investigation of HIV-induced T helper cell type 1 (Th1), Th2 and Th17
cytokine changes in the serum of treatment naive individuals. Stepwise linear discriminant
analysis (LDA) and logistic regression identified interleukin (IL)-6 to be discriminatory for HIV
infection with 74.6 and 71.2 % of the cases correctly classified. Analysis of variance
(ANOVA) confirmed IL-6 and IL-10 concentrations to be significantly (p= 0.001 and p=0.025)
different between the groups. A scatter plot of the log IL-6 and IL-10 concentrations for the
groups largely overlapped, with improved differentiation where patients were advancing to
the acquired immunodeficiency syndrome (AIDS). IL-17A levels were higher than other
cytokines but did not significantly distinguish the groups suggesting that the HIV- and HIV+
individuals had similar immune profiles. This possibility was supported by other clinical
indicators. Taken together, the measured cytokines (IL-6, 10 and 17) have potential
prognostic value. |
en_US |
dc.description.librarian |
hb2013 |
en_US |
dc.description.sponsorship |
This work was supported by the National Research Foundation, Technology
Innovation Agency, Medical Research Council and the Faculty of Natural and Agricultural
Sciences at the University of Pretoria. |
en_US |
dc.description.uri |
http://www.journals.elsevier.com/cytokine |
en_US |
dc.identifier.citation |
Williams, A, Steffens, F, Reinecke, C & Meyer, D 2013, 'The Th1/Th2/Th17 cytokine profile of HIV-infected individuals : a multivariate cytokinomics approach', Cytokine, vol. 61, no. 2, pp. 521-526. |
en_US |
dc.identifier.issn |
1043-4666 (print) |
|
dc.identifier.issn |
1096-0023 (online) |
|
dc.identifier.other |
10.1016/j.cyto.2012.11.006 |
|
dc.identifier.uri |
http://hdl.handle.net/2263/21580 |
|
dc.language.iso |
en |
en_US |
dc.publisher |
Elsevier |
en_US |
dc.rights |
© 2013 Elsevier. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Cytokine. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Cytokine, vol. 61, no. 2, 2013. DOI : 10.1016/j.cyto.2012.11.006 |
en_US |
dc.subject |
Cytometric bead array |
en_US |
dc.subject |
Flow cytometry |
en_US |
dc.subject |
Cytokinomics |
en_US |
dc.subject |
Interleukin |
en_US |
dc.subject |
Multivariate |
en_US |
dc.title |
The Th1/Th2/Th17 cytokine profile of HIV-infected individuals : a multivariate cytokinomics approach |
en_US |
dc.type |
Postprint Article |
en_US |