Continuous in vitro propagation of Cowdria ruminantium (Welgevonden stock) in a canine macrophage-monocyte cell line

Josemans, A.I.; Zweygarth, Erich

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dc.contributor.author	Josemans, A.I.
dc.contributor.editor	Boomker, Jacob Diederik Frederik
dc.contributor.upauthor	Zweygarth, Erich
dc.date.accessioned	2012-03-06T12:26:33Z
dc.date.available	2012-03-06T12:26:33Z
dc.date.created	2012
dc.date.issued	2001
dc.description	The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat v.9 was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.	en
dc.description.abstract	The Welgevonden stock of Cowdria ruminantium, aetiologic agent of heartwater, was continuously propagated in DH82 cells, a continuous canine macrophage-monocyte cell line. Cultures of DH82 cells were readily infected provided that the culture medium was supplemented with cycloheximide. Cultures were split at regular 3-day intervals and infection rates ranged between 60% and 95%. Cultures were continuously propagated through more than 125 passages over a period of more than one year.	en
dc.description.librarian	mn2012	en
dc.description.sponsorship	Agricultural Research Council of South Africa and the European Union (Cowdriosis Network) Grant no. IC18-CT95-0008 (DG12-SNRD).	en
dc.identifier.citation	Zweygarth, E & Josemans, AI 2001, 'Continuous in vitro propagation of Cowdria ruminantium (Welgevonden stock) in a canine macrophage-monocyte cell line’. Onderstepoort Journal of Veterinary Research, vol. 68, no. 2, pp. 155-157.	en
dc.identifier.issn	0030-2465
dc.identifier.uri	http://hdl.handle.net/2263/18399
dc.language	en
dc.publisher	Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria.	en
dc.rights	© ARC-Onderstepoort and Faculty of Veterinary Science, University of Pretoria (original). © University of Pretoria. Dept of Library Services (digital).	en
dc.subject	Veterinary medicine	en
dc.subject	Canine macrophage-monocyte cell line	en
dc.subject	Cowdria ruminantium	en
dc.subject	Cycloheximide	en
dc.subject	DH82	en
dc.subject	Heartwater	en
dc.subject	In vitro cultivation	en
dc.subject.lcsh	Veterinary medicine -- South Africa
dc.subject.lcsh	Tick-borne diseases in animals	en
dc.subject.lcsh	Heartwater -- South Africa	en
dc.subject.lcsh	Communicable diseases in animals	en
dc.title	Continuous in vitro propagation of Cowdria ruminantium (Welgevonden stock) in a canine macrophage-monocyte cell line	en
dc.type	Article	en