In silico secretome analyses of the polyphagous root-knot nematode Meloidogyne javanica : a resource for studying M. javanica secreted protein

dc.contributor.authorMacharia, Teresia N.
dc.contributor.authorDuong, Tuan A.
dc.contributor.authorMoleleki, Lucy N.
dc.contributor.emaillucy.moleleki@fabi.up.ac.za
dc.date.accessioned2023-11-13T09:40:49Z
dc.date.available2023-11-13T09:40:49Z
dc.date.issued2023-06
dc.descriptionDATA AVAILABILITY : All data generated or analyzed during this study are available in the figshare repository, https://doi.org/10.6084/m9.figshare.21641726.en_US
dc.description.abstractBACKGROUND: Plant-parasitic nematodes (PPNs) that cause most damage include root-knot nematodes (RKNs) which are a major impediment to crop production. Root-knot nematodes, like other parasites, secrete proteins which are required for parasite proliferation and survival within the host during the infection process. RESULTS: Here, we used various computational tools to predict and identify classically and non-classically secreted proteins encoded in the Meloidogyne javanica genome. Furthermore, functional annotation analysis was performed using various integrated bioinformatic tools to determine the biological significance of the predicted secretome. In total, 7,458 proteins were identified as secreted ones. A large percentage of this secretome is comprised of small proteins of ≤ 300 aa sequence length. Functional analyses showed that M. javanica secretome comprises cell wall degrading enzymes for facilitating nematode invasion, and migration by disintegrating the complex plant cell wall components. In addition, peptidases and peptidase inhibitors are an important category of M. javanica secretome involved in compatible host-nematode interactions. CONCLUSION: This study identifies the putative secretome encoded in the M. javanica genome. Future experimental validation analyses can greatly benefit from this global analysis of M. javanica secretome. Equally, our analyses will advance knowledge of the interaction between plants and nematodes.en_US
dc.description.departmentBiochemistryen_US
dc.description.departmentForestry and Agricultural Biotechnology Institute (FABI)en_US
dc.description.departmentGeneticsen_US
dc.description.sponsorshipThe National Research Foundation (NRF).en_US
dc.description.urihttps://bmcgenomics.biomedcentral.comen_US
dc.identifier.citationMacharia, T.N., Duong, T.A. & Moleleki, L.N. In silico secretome analyses of the polyphagous root-knot nematode Meloidogyne javanica: a resource for studying M. javanica secreted proteins. BMC Genomics 24, 296 (2023). https://doi.org/10.1186/s12864-023-09366-6.en_US
dc.identifier.issn1471-2164 (online)
dc.identifier.other10.1186/s12864-023-09366-6
dc.identifier.urihttp://hdl.handle.net/2263/93255
dc.language.isoenen_US
dc.publisherBMCen_US
dc.rights© The Author(s) 2023. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License.en_US
dc.subjectSecretome predictionen_US
dc.subjectPlant-nematode interactionen_US
dc.subjectBioinformaticsen_US
dc.subjectEffectorsen_US
dc.subjectPlant-parasitic nematode (PPN)en_US
dc.subjectRoot-knot nematode (RKN)en_US
dc.titleIn silico secretome analyses of the polyphagous root-knot nematode Meloidogyne javanica : a resource for studying M. javanica secreted proteinen_US
dc.typeArticleen_US

Files

Original bundle

Now showing 1 - 3 of 3
Loading...
Thumbnail Image
Name:
Macharia_In_2023.pdf
Size:
2.47 MB
Format:
Adobe Portable Document Format
Description:
Article
Loading...
Thumbnail Image
Name:
Macharia_InSuppl1_2023.xlsx
Size:
1.22 MB
Format:
Microsoft Excel XML
Description:
Supplementary Information 1
Loading...
Thumbnail Image
Name:
Macharia_InSuppl2_2023.docx
Size:
1.19 MB
Format:
Microsoft Word XML
Description:
Supplementary Information 2

License bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description: