Continuous in vitro propagation of Cowdria ruminantium (Welgevonden stock) in a canine macrophage-monocyte cell line

dc.contributor.authorJosemans, A.I.
dc.contributor.editorBoomker, Jacob Diederik Frederik
dc.contributor.upauthorZweygarth, Erich
dc.date.accessioned2012-03-06T12:26:33Z
dc.date.available2012-03-06T12:26:33Z
dc.date.created2012
dc.date.issued2001
dc.descriptionThe articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat v.9 was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.en
dc.description.abstractThe Welgevonden stock of Cowdria ruminantium, aetiologic agent of heartwater, was continuously propagated in DH82 cells, a continuous canine macrophage-monocyte cell line. Cultures of DH82 cells were readily infected provided that the culture medium was supplemented with cycloheximide. Cultures were split at regular 3-day intervals and infection rates ranged between 60% and 95%. Cultures were continuously propagated through more than 125 passages over a period of more than one year.en
dc.description.librarianmn2012en
dc.description.sponsorshipAgricultural Research Council of South Africa and the European Union (Cowdriosis Network) Grant no. IC18-CT95-0008 (DG12-SNRD).en
dc.identifier.citationZweygarth, E & Josemans, AI 2001, 'Continuous in vitro propagation of Cowdria ruminantium (Welgevonden stock) in a canine macrophage-monocyte cell line’. Onderstepoort Journal of Veterinary Research, vol. 68, no. 2, pp. 155-157.en
dc.identifier.issn0030-2465
dc.identifier.urihttp://hdl.handle.net/2263/18399
dc.languageen
dc.publisherPublished jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria.en
dc.rights© ARC-Onderstepoort and Faculty of Veterinary Science, University of Pretoria (original). © University of Pretoria. Dept of Library Services (digital).en
dc.subjectVeterinary medicineen
dc.subjectCanine macrophage-monocyte cell lineen
dc.subjectCowdria ruminantiumen
dc.subjectCycloheximideen
dc.subjectDH82en
dc.subjectHeartwateren
dc.subjectIn vitro cultivationen
dc.subject.lcshVeterinary medicine -- South Africa
dc.subject.lcshTick-borne diseases in animalsen
dc.subject.lcshHeartwater -- South Africaen
dc.subject.lcshCommunicable diseases in animalsen
dc.titleContinuous in vitro propagation of Cowdria ruminantium (Welgevonden stock) in a canine macrophage-monocyte cell lineen
dc.typeArticleen

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