Continuous in vitro propagation of Cowdria ruminantium (Welgevonden stock) in a canine macrophage-monocyte cell line
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Date
Authors
Josemans, A.I.
Journal Title
Journal ISSN
Volume Title
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Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria.
Abstract
The Welgevonden stock of Cowdria ruminantium, aetiologic agent of
heartwater, was continuously propagated in DH82 cells, a continuous
canine macrophage-monocyte cell line. Cultures of DH82 cells were
readily infected provided that the culture medium was supplemented with
cycloheximide. Cultures were split at regular 3-day intervals and
infection rates ranged between 60% and 95%. Cultures were continuously
propagated through more than 125 passages over a period of more than one
year.
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Keywords
Veterinary medicine, Canine macrophage-monocyte cell line, Cowdria ruminantium, Cycloheximide, DH82, Heartwater, In vitro cultivation
Sustainable Development Goals
Citation
Zweygarth, E & Josemans, AI 2001, 'Continuous in vitro propagation of Cowdria ruminantium (Welgevonden stock) in a canine macrophage-monocyte cell line’. Onderstepoort Journal of Veterinary Research, vol. 68, no. 2, pp. 155-157.