EMS derived wheat mutant BIG8-1 (Triticum aestivum L.)— a new drought tolerant mutant wheat line

dc.contributor.authorLe Roux, Marlon-Schylor L.
dc.contributor.authorBurger, Nicolas Francois V.
dc.contributor.authorVlok, Mare
dc.contributor.authorKunert, Karl J.
dc.contributor.authorCullis, Christopher A.
dc.contributor.authorBotha, Anna-Maria
dc.contributor.emailkarl.kunert@up.ac.zaen_US
dc.date.accessioned2022-04-11T10:43:36Z
dc.date.available2022-04-11T10:43:36Z
dc.date.issued2021-05-18
dc.description.abstractDrought response in wheat is considered a highly complex process, since it is a multigenic trait; nevertheless, breeding programs are continuously searching for new wheat varieties with characteristics for drought tolerance. In a previous study, we demonstrated the effectiveness of a mutant known as RYNO3936 that could survive 14 days without water. In this study, we reveal another mutant known as BIG8-1 that can endure severe water deficit stress (21 days without water) with superior drought response characteristics. Phenotypically, the mutant plants had broader leaves, including a densely packed fibrous root architecture that was not visible in the WT parent plants. During mild (day 7) drought stress, the mutant could maintain its relative water content, chlorophyll content, maximum quantum yield of PSII (Fv/Fm) and stomatal conductance, with no phenotypic symptoms such as wilting or senescence despite a decrease in soil moisture content. It was only during moderate (day 14) and severe (day 21) water deficit stress that a decline in those variables was evident. Furthermore, the mutant plants also displayed a unique preservation of metabolic activity, which was confirmed by assessing the accumulation of free amino acids and increase of antioxidative enzymes (peroxidases and glutathione S-transferase). Proteome reshuffling was also observed, allowing slow degradation of essential proteins such as RuBisCO during water deficit stress. The LC-MS/MS data revealed a high abundance of proteins involved in energy and photosynthesis under well-watered conditions, particularly Serpin-Z2A and Z2B, SGT1 and Calnexin-like protein. However, after 21 days of water stress, the mutants expressed ABC transporter permeases and xylanase inhibitor protein, which are involved in the transport of amino acids and protecting cells, respectively. This study characterizes a new mutant BIG8-1 with drought-tolerant characteristics suited for breeding programs.en_US
dc.description.departmentForestry and Agricultural Biotechnology Institute (FABI)en_US
dc.description.departmentPlant Production and Soil Scienceen_US
dc.description.librarianam2021en_US
dc.description.sponsorshipThe National Research Foundation of South Africa (NRF Competitive Programme for Rated Researchers (CPRR); NRF Incentive Funding for Rated Researchers Programme (IFR) and the Winter Cereal Trust.en_US
dc.description.urihttps://www.mdpi.com/journal/ijmsen_US
dc.identifier.citationle Roux, M.-S.L.; Burger, N.F.V.; Vlok, M.; Kunert, K.J.; Cullis, C.A.; Botha, A.-M. EMS Derived Wheat Mutant BIG8-1 (Triticum aestivum L.)—A New Drought Tolerant Mutant Wheat Line. International Journal of Molecular Sciences 2021, 22, 5314. https://DOI.org/10.3390/ijms22105314.en_US
dc.identifier.issn1661-6596 (print)
dc.identifier.issn1422-0067 (online)
dc.identifier.other10.3390/ijms22105314
dc.identifier.urihttps://repository.up.ac.za/handle/2263/84869
dc.language.isoenen_US
dc.publisherMDPI Publishingen_US
dc.rights© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.en_US
dc.subjectWater deficit stressen_US
dc.subjectProteomeen_US
dc.subjectPhysiological responsesen_US
dc.subjectEnhanced water deficit stress toleranceen_US
dc.titleEMS derived wheat mutant BIG8-1 (Triticum aestivum L.)— a new drought tolerant mutant wheat lineen_US
dc.typeArticleen_US

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