Phylogenetic characterization of the Palyam serogroup orbiviruses
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Date
Authors
Ebersohn, Karen
Coetzee, Peter
Snyman, Louwrens Pieter
Swanepoel, Robert
Venter, Estelle Hildegard
Journal Title
Journal ISSN
Volume Title
Publisher
MDPI Publishing
Abstract
The Palyam serogroup orbiviruses are associated with abortion and teratogenesis in cattle and other ruminants. Of the 13 different serotypes that have been identified, the full genome sequence of only one, Kasba, has been published. We undertook to perform Next Generation Sequencing (NGS) and phylogenetic analysis on 12 Palyam serotypes plus field isolates of the African serotypes in our possession. The Palyam serogroup was found to be most closely related to the African horse sickness virus group and showed the most distant evolutionary relationship to the equine encephalosis viruses (EEV). Amino acid sequence analysis revealed that the gene encoding VP7 was the most conserved within serotypes and VP2 and VP5 showed the highest degree of variation. A high degree of sequence identity was found for isolates from the same geographical region. The phylogenetic analysis revealed two clades where the African serotypes were all very closely related in one clade and the other clade contained the Australian and Asian serotypes and one African serotype, Petevo. It was evident from the sequence data that the geographical origin of Palyam serogroup viruses played an important role in the development of the different serotypes.
Description
Supplementary material: Table S1: Models selected by jModel test for specified gene regions. Table S2: Amino acid percentage identities for Segment 1 (VP1) on the bottom left and Segment 3 (VP3) on the top right. Table S3: Amino acid percentage identities for Segment 4 (VP4) on the bottom left and Segment 6 (VP5) on the top right. Table S4: Amino acid percentage identities for Segment 9 (VP6) on the bottom left and Segment 5 (NS1) on the top right. Table S5: Amino acid percentage identities for Segment 8 (NS2) on the bottom left and Segment 10 (NS3) on the top right.
Keywords
Sequencing, Phylogenetic analysis, Palyam serogroup orbiviruses, Next generation sequencing (NGS), Equine encephalosis viruses (EEV)
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Citation
Ebersohn, K., Coetzee, P., Snyman, L.P. et al. 2019, 'Phylogenetic characterization of the Palyam serogroup orbiviruses', Viruses, vol. 11, art. 446, pp. 1-13.