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dc.contributor.author | Madzingira, Oscar![]() |
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dc.contributor.author | Aikukutu, Gottlieb![]() |
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dc.contributor.author | Kandongo, Fidelis![]() |
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dc.contributor.author | Kolo, Francis Babaman![]() |
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dc.contributor.author | Khaiseb, Siegfried![]() |
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dc.contributor.author | Tjipura-Zaire, Georgina![]() |
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dc.contributor.author | Kabajani, Juliet N.![]() |
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dc.contributor.author | Musilika-Shilongo, Albertina![]() |
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dc.contributor.author | Van Heerden, Henriette![]() |
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dc.date.accessioned | 2024-07-30T04:38:01Z | |
dc.date.available | 2024-07-30T04:38:01Z | |
dc.date.issued | 2023 | |
dc.description | DATA AVAILABILITY STATEMENT : The datasets used and/or analysed during the current study are included in this manuscript. | en_US |
dc.description.abstract | Brucellosis is a worldwide zoonosis that is endemic in Namibia. This study estimated seroprevalence of brucellosis, and determined the presence of Brucella infection in slaughtered cattle using the genus-specific 16-23S rRNA interspacer PCR (ITS-PCR), and the species-specific AMOS-PCR. Between December 2018 and May 2019, sera (n = 304), pooled lymph nodes (n = 304), and individual spleen (n = 304) were collected from slaughtered cattle from 52 farms. Sera were tested for anti-Brucella antibodies using the Rose Bengal test (RBT), and the complement fixation test (CFT). Seroprevalence was 2.3% (7/304) (RBT) and 1.6% (5/304) (CFT). Prevalence of positive herds was 9.6% (5/52). Lymph node (n = 200) and spleen (n = 200) samples from seronegative cattle tested negative for Brucella spp. DNA on ITS-PCR, but Brucella spp. DNA was detected in lymph nodes (85.7%, 6/7) and spleen (85.7%, 6/7) from RBT positive cattle. ITS-PCR confirmed isolates from lymph node (51.4%, 4/7) and spleen (85.7%, 6/7) as Brucella spp.; while AMOS-PCR and Brucella abortus species specific (BaSS) PCR confirmed the isolates as Brucella abortus, and field strains, respectively. Provision of adequate protective gear, and the promotion of brucellosis awareness among abattoir workers is recommended to prevent zoonotic infection. | en_US |
dc.description.department | Veterinary Tropical Diseases | en_US |
dc.description.librarian | am2024 | en_US |
dc.description.sdg | SDG-02:Zero Hunger | en_US |
dc.description.sdg | SDG-03:Good heatlh and well-being | en_US |
dc.description.sponsorship | The Institute of Tropical Medicine (ITM), Antwerp (Belgium) partly funded the laboratory reagents. | en_US |
dc.description.uri | https://www.tandfonline.com/journals/ziee20 | en_US |
dc.identifier.citation | Madzingira, O., Aikukutu, G., Kandongo, F. et al. 2023, 'Seroprevalence, and molecular detection of Brucella abortus in cattle tissues from an abattoir in Namibia', Infection Ecology & Epidemiology, vol. 13, no. 1, art. 2229589, pp. 1-10. https://DOI.org/10.1080/20008686.2023.2229589. | en_US |
dc.identifier.issn | 2000-8686 | |
dc.identifier.other | 10.1080/20008686.2023.2229589 | |
dc.identifier.uri | http://hdl.handle.net/2263/97295 | |
dc.language.iso | en | en_US |
dc.publisher | Taylor and Francis | en_US |
dc.rights | © 2023 The Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License. | en_US |
dc.subject | Seroprevalence | en_US |
dc.subject | Abattoir | en_US |
dc.subject | Cattle | en_US |
dc.subject | ITS-PCR | en_US |
dc.subject | AMOS-PCR | en_US |
dc.subject | Brucella abortus | en_US |
dc.subject | SDG-02: Zero hunger | en_US |
dc.subject | SDG-03: Good health and well-being | en_US |
dc.title | Seroprevalence, and molecular detection of Brucella abortus in cattle tissues from an abattoir in Namibia | en_US |
dc.type | Article | en_US |