dc.contributor.author |
Olaogun, Sunday Charles
|
|
dc.contributor.author |
Byaruhanga, Charles
|
|
dc.contributor.author |
Ochai, Sunday Ochonu
|
|
dc.contributor.author |
Fosgate, Geoffrey Theodore
|
|
dc.contributor.author |
Marufu, Munyaradzi Christopher
|
|
dc.date.accessioned |
2023-10-03T11:13:34Z |
|
dc.date.available |
2023-10-03T11:13:34Z |
|
dc.date.issued |
2022-11-23 |
|
dc.description |
DATA AVAILABILITY STATEMENT : The data presented in this study are available on request from the corresponding author. |
en_US |
dc.description.abstract |
Fasciolosis causes significant economic losses in commercial cattle herds in South Africa, but its prevalence is unknown in most communal areas. A cross-sectional study was conducted with the aim of determining the occurrence of bovine fasciolosis using three different diagnostic methods in Moretele Local Municipality in Bojanala District, NorthWest Province. Faecal samples were collected from 277 cattle of different breeds, ages, sex and faecal condition scores and examined using the sedimentation technique, quantitative real-time polymerase chain reaction (qPCR) and faecal antigen enzyme-linked immunosorbent assay (coproELISA). All samples were negative for bovine fasciolosis using coproELISA. A total of 73 (26.4%) samples were positive using the qPCR, while 36 were positive using the sedimentation technique, with low faecal egg counts (1 to 20 eggs per gram). The qPCR detected the highest positivity (26.4%, 95% CI 21.3, 32.0) followed by the sedimentation test (13.0%; 95% CI 9.3, 17.5). Location, breed, sex, age and faecal consistency score were not associated with positive qPCR results (p > 0.05). There was also no significant agreement (kappa = 0.011, p = 0.843) between qPCR and the sedimentation technique for the detection of Fasciola spp. The qPCR appeared to be the most sensitive method for detection of Fasciola spp. Further studies are required on the characterisation of Fasciola spp. in communal cattle in South Africa. |
en_US |
dc.description.department |
Production Animal Studies |
en_US |
dc.description.department |
Veterinary Tropical Diseases |
en_US |
dc.description.librarian |
am2023 |
en_US |
dc.description.sponsorship |
The National Research Foundation (NRF) of South Africa, the University of Pretoria (South Africa) postgraduate bursary and the University of Ibadan (Nigeria) Tertiary Education Trust Fund. |
en_US |
dc.description.uri |
https://www.mdpi.com/journal/pathogens |
en_US |
dc.identifier.citation |
Olaogun, S.C.;
Byaruhanga, C.; Ochai, S.O.;
Fosgate, G.T.; Marufu, M.C.
Comparison of Three Diagnostic
Methods to Detect the Occurrence of
Fasciola Species in Communally
Grazed Cattle in the NorthWest
Province, South Africa. Pathogens
2022, 11, 1398. https://DOI.org/10.3390/pathogens11121398. |
en_US |
dc.identifier.issn |
2076-2607 (online) |
|
dc.identifier.other |
10.3390/pathogens11121398 |
|
dc.identifier.uri |
http://hdl.handle.net/2263/92668 |
|
dc.language.iso |
en |
en_US |
dc.publisher |
MDPI |
en_US |
dc.rights |
© 2022 by the authors.
Licensee MDPI, Basel, Switzerland.
This article is an open access article
distributed under the terms and
conditions of the Creative Commons
Attribution (CC BY) license. |
en_US |
dc.subject |
Antigen ELISA |
en_US |
dc.subject |
Bovine |
en_US |
dc.subject |
Fasciola species |
en_US |
dc.subject |
Real-time PCR |
en_US |
dc.subject |
Sedimentation |
en_US |
dc.subject |
Polymerase chain reaction (PCR) |
en_US |
dc.subject |
Quantitative polymerase chain reaction (qPCR) |
en_US |
dc.subject |
Faecal antigen enzyme-linked immunosorbent assay (coproELISA) |
en_US |
dc.subject |
Enzyme-linked immunosorbent assay (ELISA) |
en_US |
dc.subject |
Fasciola species |
en_US |
dc.subject |
North West Province, South Africa |
en_US |
dc.title |
Comparison of three diagnostic methods to detect the occurrence of Fasciola species in communally grazed cattle in the North West Province, South Africa |
en_US |
dc.type |
Article |
en_US |