A series of novel tetrazine derivatives, containing benzothiazole framework, were prepared during the
coupling reactions of some diazotized 2-aminobenzo[d]thiazole derivatives with p-acetaminophen. Their
structures were elucidated based on NMR and MS spectrometry. The anticancer activity and the safety of the
synthesized compounds along with the entire precursors were assessed against three human cancer cell lines
and a normal cell line. All the synthesized compounds showed selective cytotoxic activity against the cancer
cell lines used in comparison to the normal Vero cell line. Their IC50 values varied from 2.02 to 171.67 μM.
