dc.contributor.author |
Mashamba, Tebatso Gratitude
|
|
dc.contributor.author |
Adeosun, Idowu Jesulayomi
|
|
dc.contributor.author |
Baloyi, Itumeleng Tsebang
|
|
dc.contributor.author |
Tshikalange, Thilivhali Emmanuel
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|
dc.contributor.author |
Cosa, Sekelwa
|
|
dc.date.accessioned |
2022-11-10T05:34:50Z |
|
dc.date.available |
2022-11-10T05:34:50Z |
|
dc.date.issued |
2022-04-27 |
|
dc.description.abstract |
The crisis of antibiotic resistance necessitates the search of phytochemicals as potential antibacterial, anti-quorum sensing and antibiofilm forming agents. For the present study, fifteen (15) selected medicinal plants were evaluated to inhibit the biological activities of multi-drug resistant (MDR) pathogenic bacteria (Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, and Proteus mirabilis) associated with diabetic foot ulcer. Antibacterial activities revealed noteworthy minimum inhibitory concentration (MIC) values ≤1 mg/mL for thirteen (13) out of the sixty (60) plant extracts screened. The potent extracts included Euclea natalensis ethyl acetate (0.25 mg/mL), Aloe ferox methanol (0.5 mg/ml) and Warburgia salutaris aqueous (0.5 mg/mL) extracts. Chemical profiling of the active extracts using gas chromatography-mass spectrometry (GC-MS) identified neophytadiene, guanosine, squalene, cis megastigma-5,8-diene-4-one and sorbitol as prevalent compounds among the active extracts. Anti-quorum sensing activities of E. natalensis (ethyl acetate), A. ferox (methanol) and W. salutaris (aqueous) extracts ranged from 4.81 - 58.34% with E. natalensis (ethyl-acetate) showing the highest activity. Molecular docking against CviR protein showed selected compounds having high docking scores with sorbitol showing the highest score of -7.04 kcal/mol. Warburgia salutaris aqueous extract exhibited the highest biofilm inhibition (73%) against E. coli. Euclea natalensis, Aloe ferox and Warburgia salutaris compounds act as antagonist of N-acyl homoserine lactone (AHL) signaling, thus may serve as candidates in antipathogenic and antibiofilm phytomedicine development for MDR foot ulcer bacterial pathogens. |
en_US |
dc.description.department |
Biochemistry |
en_US |
dc.description.department |
Genetics |
en_US |
dc.description.department |
Microbiology and Plant Pathology |
en_US |
dc.description.department |
Plant Production and Soil Science |
en_US |
dc.description.sponsorship |
The South African National Research Foundation (NRF) Thuthuka Grant and the South African Medical Research Council-Self Initiated Research (SAMRC-SIR). |
en_US |
dc.description.uri |
https://www.cell.com/heliyon/home |
en_US |
dc.identifier.citation |
Mashamba, T.G., Adeosun, I.J., Baloyi, I.T., Tshikalange, E.T. & Cosa S.
Quorum sensing modulation and inhibition in biofilm forming foot ulcer pathogens by selected medicinal plants. Heliyon. 2022 Apr 27;8(4):e09303. doi:10.1016/j.heliyon.2022.e09303. |
en_US |
dc.identifier.issn |
2405-8440 (online) |
|
dc.identifier.other |
10.1016/j.heliyon.2022.e09303 |
|
dc.identifier.uri |
https://repository.up.ac.za/handle/2263/88235 |
|
dc.language.iso |
en |
en_US |
dc.publisher |
Elsevier |
en_US |
dc.rights |
© 2022 Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
en_US |
dc.subject |
Aloe ferox |
en_US |
dc.subject |
Antiquorum sensing |
en_US |
dc.subject |
Biofilm inhibition |
en_US |
dc.subject |
Euclea natalensis |
en_US |
dc.subject |
Molecular modelling |
en_US |
dc.subject |
Multi-drug resistant pathogens |
en_US |
dc.subject |
Multidrug-resistant (MDR) |
en_US |
dc.title |
Quorum sensing modulation and inhibition in biofilm forming foot ulcer pathogens by selected medicinal plants |
en_US |
dc.type |
Article |
en_US |