SUPPLEMENTARY MATERIAL : FIGURE S1. Metabolite distribution of Rhizopus delemar with the minor
components including biomass, succinic acid and pyruvic acid. Cultures were incubated at 34 °C,
200 rpm in the presence of 20 g/L CaCO3
. FIGURE S2. Metabolite distribution of Rhizopus delemar with
the minor components including biomass, succinic acid and pyruvic acid. Cultures were incubated
at 34 °C, 200 rpm in the presence of 60 g/L CaCO3
. FIGURE S3. Metabolite distribution of Rhizopus
delemar with the minor components including biomass, succinic acid and pyruvic acid. Cultures were
incubated at 34 °C, 200 rpm in the presence of 60 g/L CaCO3
. FIGURE S4. Extracellular concentrations
of glycerol during shake ask cultivation of R. delemar with diferent plaster sand concentrations. Results
are the mean of triplicate experiments and error bars indicate the standard deviation. FIGURE S5.
Extracellular concentrations of glycerol during shake ask cultivation of R. delemar with different
calcium ion concentrations. 20 g L−1 CaCO3 was used for pH control as a baseline amount in these
experiments and additional 5, 10 and 20 g L−1
calcium ions were added onto the baseline in the form
of CaCl3
.
DATA AVAILABITY STATEMENT : The data presented in this study are openly available in the University
of Pretoria Research Data Repository at DOI: 10.25403/UPresearchdata.19229979.