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dc.contributor.author | Ebersohn, Karen![]() |
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dc.contributor.author | Coetzee, Peter![]() |
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dc.contributor.author | Snyman, Louwrens Pieter![]() |
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dc.contributor.author | Swanepoel, Robert![]() |
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dc.contributor.author | Venter, Estelle Hildegard![]() |
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dc.date.accessioned | 2021-01-06T12:01:27Z | |
dc.date.available | 2021-01-06T12:01:27Z | |
dc.date.issued | 2019-05 | |
dc.description | Supplementary material: Table S1: Models selected by jModel test for specified gene regions. Table S2: Amino acid percentage identities for Segment 1 (VP1) on the bottom left and Segment 3 (VP3) on the top right. Table S3: Amino acid percentage identities for Segment 4 (VP4) on the bottom left and Segment 6 (VP5) on the top right. Table S4: Amino acid percentage identities for Segment 9 (VP6) on the bottom left and Segment 5 (NS1) on the top right. Table S5: Amino acid percentage identities for Segment 8 (NS2) on the bottom left and Segment 10 (NS3) on the top right. | en_ZA |
dc.description.abstract | The Palyam serogroup orbiviruses are associated with abortion and teratogenesis in cattle and other ruminants. Of the 13 different serotypes that have been identified, the full genome sequence of only one, Kasba, has been published. We undertook to perform Next Generation Sequencing (NGS) and phylogenetic analysis on 12 Palyam serotypes plus field isolates of the African serotypes in our possession. The Palyam serogroup was found to be most closely related to the African horse sickness virus group and showed the most distant evolutionary relationship to the equine encephalosis viruses (EEV). Amino acid sequence analysis revealed that the gene encoding VP7 was the most conserved within serotypes and VP2 and VP5 showed the highest degree of variation. A high degree of sequence identity was found for isolates from the same geographical region. The phylogenetic analysis revealed two clades where the African serotypes were all very closely related in one clade and the other clade contained the Australian and Asian serotypes and one African serotype, Petevo. It was evident from the sequence data that the geographical origin of Palyam serogroup viruses played an important role in the development of the different serotypes. | en_ZA |
dc.description.department | Veterinary Tropical Diseases | en_ZA |
dc.description.librarian | am2020 | en_ZA |
dc.description.sponsorship | The National Research Foundation, EH Venter Incentive Funds and the University of Pretoria. L.S. acknowledge the Belgian Directorate-General for the Development Co-operation Framework Agreement (FA4 DGD-ITM 2017-2021) awarded to the Department of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, as well as a DST/NRF freestanding award (116711) as post-doctoral funding sources. | en_ZA |
dc.description.uri | http://www.mdpi.com/journal/viruses | en_ZA |
dc.identifier.citation | Ebersohn, K., Coetzee, P., Snyman, L.P. et al. 2019, 'Phylogenetic characterization of the Palyam serogroup orbiviruses', Viruses, vol. 11, art. 446, pp. 1-13. | en_ZA |
dc.identifier.issn | 1999-4915 (online) | |
dc.identifier.other | 10.3390/v11050446 | |
dc.identifier.uri | http://hdl.handle.net/2263/77947 | |
dc.language.iso | en | en_ZA |
dc.publisher | MDPI Publishing | en_ZA |
dc.rights | © 2019 by the authors. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license. | en_ZA |
dc.subject | Sequencing | en_ZA |
dc.subject | Phylogenetic analysis | en_ZA |
dc.subject | Palyam serogroup orbiviruses | en_ZA |
dc.subject | Next generation sequencing (NGS) | en_ZA |
dc.subject | Equine encephalosis viruses (EEV) | en_ZA |
dc.title | Phylogenetic characterization of the Palyam serogroup orbiviruses | en_ZA |
dc.type | Article | en_ZA |